User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/20 - Revision history

Keyun Wang at 19:22, 27 November 2012

Keyun Wang — Tue, 27 Nov 2012 19:22:32 GMT

←Older revision		Revision as of 19:22, 27 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	~~==Purpose==~~
-	* ADA protein were transferred from dialysis tubing into 15mL falcon tubes
-	* Make Au/ADA samples with the following mole ratios: 60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150, with ADA fraction 2 after dialysis.
-	* Un-dialyzed Au/ADA samples and Au/HRP made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]] were run on UV-vis spectrometer and Atomic Absorption Spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.
-	* Resuspend Au/HRP samples in different concentration and pH of Tris buffer to test ionic strength.
-
-	~~==Procedure for Dialysis==~~
-	* Dialysis beaker containing dialysis tubing enclosed with ADA protein fractions were taken from the 4°C cold room into room temperature lab room.
-	* The dialysis clips were taken off from dialysis tubing. ADA protein fractions were poured into a sterile 15mL falcon tubes.
-	* This process was repeated for all three protein fractions: ADA fraction 1&3 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06\|2012/11/06]], ADA fraction 2 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06\|2012/11/06]], and ADA fraction purified on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03\|2012/10/03]].
-	* ADA fractions in 15mL falcon tubes were stored in 4°C refrigerator.
-
-	~~==Procedure for making dialyzed Au/ADA samples==~~
-	* Au/ADA samples were made with the following mole ratios:
-	~~60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150~~
-	* Stock solution of HAuCl<sub>4</sub> was made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]] with a concentration of 10.5uM.
-	* Stock solution for ADA was the ADA protein fraction made in [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03\|2012/10/03]] after dialysis. The ADA stock solution has a concentration of 58.36μM.
-	* Volume of ADA protein was set at 137.1uL and a range of HAuCl<sub>4</sub> was used from 45.71μL to 114.3μL. Water was added to the sample to increase the volume of sample to 8mL. Volumes of each reactants are shown in table below:
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Au/ADA ratio'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''ADA added[uL]'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''HAuCl4 Added [uL]'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Water Added[uL]'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[ADA]final[uM]'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[HAuCl4]final[uM]'''~~
-	\|-
-	~~\| 60\|\|137.1\|\|45.71\|\|7817.2\|\|1\|\|60~~
-	\|-
-	~~\| 70\|\|137.1\|\|53.3\|\|7809.6\|\|1\|\|70~~
-	\|-
-	~~\| 80\|\|137.1\|\|60.9\|\|7802\|\|1\|\|80~~
-	\|-
-	~~\| 90\|\|137.1\|\|68.6\|\|7794.4\|\|1\|\|90~~
-	\|-
-	~~\| 100\|\|137.1\|\|76.2\|\|7786.8\|\|1\|\|100~~
-	\|-
-	~~\| 110\|\|137.1\|\|83.8\|\|7779.1\|\|1\|\|110~~
-	\|-
-	~~\| 120\|\|137.1\|\|91.4\|\|7771.5\|\|1\|\|120~~
-	\|-
-	~~\| 130\|\|137.1\|\|99\|\|7763.9\|\|1\|\|130~~
-	\|-
-	~~\| 140\|\|137.1\|\|106.7\|\|7756.3\|\|1\|\|140~~
-	\|-
-	~~\| 150\|\|137.1\|\|114.3\|\|7748.7\|\|1\|\|150~~
-	\|-
-	\|
-	\|}
-
-	* The samples were made in 15mL non-sterile falcon tubes. After all reactants were added, samples are capped and wrapped around with aluminum foil.
-	* Samples were placed in incubator at 85°C for 4 hours then cooled down to room temperature.
-
-	~~==Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples==~~
-	* UV-vis spectrometer were run in spectrum method. A range of 200nm to 800nm were ran on all Au/ADA and Au/HRP samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]].
-	* 3mL of distilled water was placed in quartz cuvette with 1cm pathlength and set as base line solution. 3mL taken from supernatant of Au/ADA and Au/HRP samples were placed in an identical quartz cuvette with 1cm pathlength for spectra measurement.
-
-	~~==Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples==~~

		+	Nothing was done.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Keyun Wang: /* Procedure for Running UV-vis Spectrometer and AA Spectrometer on Au/ADA and Au/HRP samples */

Keyun Wang — Tue, 27 Nov 2012 19:20:50 GMT

Procedure for Running UV-vis Spectrometer and AA Spectrometer on Au/ADA and Au/HRP samples

←Older revision		Revision as of 19:20, 27 November 2012
Line 58:		Line 58:
	* Samples were placed in incubator at 85°C for 4 hours then cooled down to room temperature.		* Samples were placed in incubator at 85°C for 4 hours then cooled down to room temperature.

-	==Procedure for Running UV-vis Spectrometer and AA Spectrometer on Au/ADA and Au/HRP samples==	+	==Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples==
		+	* UV-vis spectrometer were run in spectrum method. A range of 200nm to 800nm were ran on all Au/ADA and Au/HRP samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]].
		+	* 3mL of distilled water was placed in quartz cuvette with 1cm pathlength and set as base line solution. 3mL taken from supernatant of Au/ADA and Au/HRP samples were placed in an identical quartz cuvette with 1cm pathlength for spectra measurement.
		+
		+	==Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples==
		+

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Keyun Wang: /* Procedure for Running UV-vis spectrometer and Atomic Absorption spectrometer on Au/ADA and Au/HRP samples */

Keyun Wang — Tue, 27 Nov 2012 19:15:12 GMT

Procedure for Running UV-vis spectrometer and Atomic Absorption spectrometer on Au/ADA and Au/HRP samples

←Older revision		Revision as of 19:15, 27 November 2012
Line 58:		Line 58:
	* Samples were placed in incubator at 85°C for 4 hours then cooled down to room temperature.		* Samples were placed in incubator at 85°C for 4 hours then cooled down to room temperature.

-	==Procedure for Running UV-vis ~~spectrometer~~ and ~~Atomic Absorption spectrometer~~ on Au/ADA and Au/HRP samples==	+	==Procedure for Running UV-vis Spectrometer and AA Spectrometer on Au/ADA and Au/HRP samples==

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Keyun Wang: /* Purpose */

Keyun Wang — Tue, 27 Nov 2012 19:14:36 GMT

Purpose

←Older revision		Revision as of 19:14, 27 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Purpose==		==Purpose==
		+	* ADA protein were transferred from dialysis tubing into 15mL falcon tubes
	* Make Au/ADA samples with the following mole ratios: 60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150, with ADA fraction 2 after dialysis.		* Make Au/ADA samples with the following mole ratios: 60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150, with ADA fraction 2 after dialysis.
-	* ~~Run un~~-dialyzed Au/ADA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]] on UV-vis spectrometer and Atomic Absorption Spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.	+	* Un-dialyzed Au/ADA samples and Au/HRP made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]] were run on UV-vis spectrometer and Atomic Absorption Spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.
-	*	+	* Resuspend Au/HRP samples in different concentration and pH of Tris buffer to test ionic strength.

		+	==Procedure for Dialysis==
		+	* Dialysis beaker containing dialysis tubing enclosed with ADA protein fractions were taken from the 4°C cold room into room temperature lab room.
		+	* The dialysis clips were taken off from dialysis tubing. ADA protein fractions were poured into a sterile 15mL falcon tubes.
		+	* This process was repeated for all three protein fractions: ADA fraction 1&3 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06\|2012/11/06]], ADA fraction 2 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06\|2012/11/06]], and ADA fraction purified on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03\|2012/10/03]].
		+	* ADA fractions in 15mL falcon tubes were stored in 4°C refrigerator.

		+	==Procedure for making dialyzed Au/ADA samples==
		+	* Au/ADA samples were made with the following mole ratios:
		+	60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
		+	* Stock solution of HAuCl<sub>4</sub> was made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]] with a concentration of 10.5uM.
		+	* Stock solution for ADA was the ADA protein fraction made in [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03\|2012/10/03]] after dialysis. The ADA stock solution has a concentration of 58.36μM.
		+	* Volume of ADA protein was set at 137.1uL and a range of HAuCl<sub>4</sub> was used from 45.71μL to 114.3μL. Water was added to the sample to increase the volume of sample to 8mL. Volumes of each reactants are shown in table below:
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Au/ADA ratio'''
		+	\| align="center" style="background:#f0f0f0;"\|'''ADA added[uL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''HAuCl4 Added [uL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Water Added[uL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''[ADA]final[uM]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''[HAuCl4]final[uM]'''
		+	\|-
		+	\| 60\|\|137.1\|\|45.71\|\|7817.2\|\|1\|\|60
		+	\|-
		+	\| 70\|\|137.1\|\|53.3\|\|7809.6\|\|1\|\|70
		+	\|-
		+	\| 80\|\|137.1\|\|60.9\|\|7802\|\|1\|\|80
		+	\|-
		+	\| 90\|\|137.1\|\|68.6\|\|7794.4\|\|1\|\|90
		+	\|-
		+	\| 100\|\|137.1\|\|76.2\|\|7786.8\|\|1\|\|100
		+	\|-
		+	\| 110\|\|137.1\|\|83.8\|\|7779.1\|\|1\|\|110
		+	\|-
		+	\| 120\|\|137.1\|\|91.4\|\|7771.5\|\|1\|\|120
		+	\|-
		+	\| 130\|\|137.1\|\|99\|\|7763.9\|\|1\|\|130
		+	\|-
		+	\| 140\|\|137.1\|\|106.7\|\|7756.3\|\|1\|\|140
		+	\|-
		+	\| 150\|\|137.1\|\|114.3\|\|7748.7\|\|1\|\|150
		+	\|-
		+	\|
		+	\|}
		+
		+	* The samples were made in 15mL non-sterile falcon tubes. After all reactants were added, samples are capped and wrapped around with aluminum foil.
		+	* Samples were placed in incubator at 85°C for 4 hours then cooled down to room temperature.
		+
		+	==Procedure for Running UV-vis spectrometer and Atomic Absorption spectrometer on Au/ADA and Au/HRP samples==

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Keyun Wang: /* Purpose */

Keyun Wang — Tue, 27 Nov 2012 17:39:10 GMT

Purpose

←Older revision		Revision as of 17:39, 27 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Purpose==		==Purpose==
-	* Make Au/ADA samples with the following mole ratios: ~~_____~~ with ADA fraction 2 after dialysis.	+	* Make Au/ADA samples with the following mole ratios: 60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150, with ADA fraction 2 after dialysis.
	* Run un-dialyzed Au/ADA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]] on UV-vis spectrometer and Atomic Absorption Spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.		* Run un-dialyzed Au/ADA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]] on UV-vis spectrometer and Atomic Absorption Spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.
	*		*

Keyun Wang at 17:38, 27 November 2012

Keyun Wang — Tue, 27 Nov 2012 17:38:34 GMT

←Older revision		Revision as of 17:38, 27 November 2012
Line 8:		Line 8:
	==Purpose==		==Purpose==
	* Make Au/ADA samples with the following mole ratios: _____ with ADA fraction 2 after dialysis.		* Make Au/ADA samples with the following mole ratios: _____ with ADA fraction 2 after dialysis.
-	* Run un-dialyzed Au/ADA samples made on ~~_____date~~ on UV-vis spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.	+	* Run un-dialyzed Au/ADA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14\|2012/11/14]] on UV-vis spectrometer and Atomic Absorption Spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.
	*		*

Keyun Wang at 17:36, 27 November 2012

Keyun Wang — Tue, 27 Nov 2012 17:36:31 GMT

←Older revision		Revision as of 17:36, 27 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Purpose==		==Purpose==
		+	* Make Au/ADA samples with the following mole ratios: _____ with ADA fraction 2 after dialysis.
		+	* Run un-dialyzed Au/ADA samples made on _____date on UV-vis spectrometer in order to compare spectra results for Au/ADA, Au/HRP, Au/Lysozyme, and Au/BSA.
	*		*
		+

Keyun Wang at 17:31, 27 November 2012

Keyun Wang — Tue, 27 Nov 2012 17:31:56 GMT

←Older revision		Revision as of 17:31, 27 November 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here...~~	+	*

Keyun Wang: Autocreate 2012/11/20 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

Keyun Wang — Tue, 27 Nov 2012 17:28:57 GMT

Autocreate 2012/11/20 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__