User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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** All three dialysis tubes were placed in water in dialysis beaker with stir bar. | ** All three dialysis tubes were placed in water in dialysis beaker with stir bar. | ||
** Dialysis beaker was covered with aluminum foil to prevent loss of water in the process, and the beaker was placed on a stir plate in -40°C for 12 days. | ** Dialysis beaker was covered with aluminum foil to prevent loss of water in the process, and the beaker was placed on a stir plate in -40°C for 12 days. | ||
** Distilled water in dialysis beaker was changed after two days, and dialysis tubings were soaked in new distilled water for 10 days. | |||
* The Au/ADA solutions were made to ensure the ratio of fibers formed. The ratios of Au/ADA solutions were as follows: | * The Au/ADA solutions were made to ensure the ratio of fibers formed. The ratios of Au/ADA solutions were as follows: | ||
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| 150||383.7||114.3||7502.0 | | 150||383.7||114.3||7502.0 | ||
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* Above samples were also placed in incubator with same protocol as that of Au/ADA and Au/HRP samples. | * Above samples were also placed in incubator with same protocol as that of Au/ADA and Au/HRP samples. | ||
==Notes== | ==Notes== |
Latest revision as of 22:15, 26 September 2017
Experimental Biological Chemistry I | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
130 - 140 - 150
Absorbance = Molar absorptivity * Path length * Concentration 2.100 = 102 M*cm-1 * 1cm * Concentration Concentration of HRP sample = 20.58uM
Notes
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