User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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* In the graph above, the concentration of gold in Au/Lysozyme supernatant increased from mole ratio of 20 to 60, and dropped immediately from mole ratio of 60 and on. From mole ratio of 60 and on, there are no gold present in supernatant. The samples with mole ratio from 60 and on appear transparent as oppose to purple. | * In the graph above, the concentration of gold in Au/Lysozyme supernatant increased from mole ratio of 20 to 60, and dropped immediately from mole ratio of 60 and on. From mole ratio of 60 and on, there are no gold present in supernatant. The samples with mole ratio from 60 and on appear transparent as oppose to purple. | ||
* This result indicates that protein started to form aggregation from mole ratio of 60 and on. Compare to the result with Au/HRP from above and Au/BSA from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17|2012/10/17]], the behavior of Au/Lysozyme resemble more like Au/BSA samples in terms of protein aggregation formation. This result also indicates that BSA and lysozyme behavior similarly when forming gold nano particles. It can also indicate that they have similar protein folding and unfolding kinetic mechanism. | * This result indicates that protein started to form aggregation from mole ratio of 60 and on. Compare to the result with Au/HRP from above and Au/BSA from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17|2012/10/17]], the behavior of Au/Lysozyme resemble more like Au/BSA samples in terms of protein aggregation formation. This result also indicates that BSA and lysozyme behavior similarly when forming gold nano particles. It can also indicate that they have similar protein folding and unfolding kinetic mechanism. | ||
* Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13|Melissa Novy's Notebook]] for graph and data plotting the percent concentration change before and after in unit of ppm for both Au/HRP and Au/Lysozyme. | |||
==Procedure on making adenosine== | ==Procedure on making adenosine== |
Revision as of 21:25, 7 December 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure on UV-vis spectrometry of Au/HRP
10 - 50 - 100 - 150 - 200 - 250 - 300 - 350 - 400 - 450
Data on UV-vis spectrometry of Au/HRP
Procedure on Atomic Absorption Spectrometry of Au/lysozyme and Au/HRP solutions
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions
Procedure on making adenosine
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