User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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* The pH of sodium phosphate buffer was adjusted to 7.4 using HCl. | * The pH of sodium phosphate buffer was adjusted to 7.4 using HCl. | ||
* 0.1mM of adenosine was made in 5mL of sodium phosphate buffer. | * 0.1mM of adenosine was made in 5mL of sodium phosphate buffer. | ||
* For details of the calculations, see [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13|Melissa's Notebook]], which includes calculations for making sodium phosphate buffer, pH adjustment of sodium phosphate buffer, calculations to weight out the adenosine, and calculations on the amount of sodium phosphate buffer to add to adenosine in order to reach 0.1mM adenosine. | * This procedure was not done by writer, thus was not described in details. For details of the calculations, see [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13|Melissa's Notebook]], which includes calculations for making sodium phosphate buffer, pH adjustment of sodium phosphate buffer, calculations to weight out the adenosine, and calculations on the amount of sodium phosphate buffer to add to adenosine in order to reach 0.1mM adenosine. | ||
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Revision as of 20:34, 23 November 2012
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Purpose
Procedure on UV-vis spectrometry of Au/HRP
10 - 50 - 100 - 150 - 200 - 250 - 300 - 350 - 400 - 450
Data on UV-vis spectrometry of Au/HRP
Procedure on Atomic Absorption Spectrometry of Au/lysozyme and Au/HRP solutions
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions
Procedure on making adenosine
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