User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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* It was concluded to test ratios between 50 to 150 in smaller increments for more accurate conclusion on when protein aggregates form. | * It was concluded to test ratios between 50 to 150 in smaller increments for more accurate conclusion on when protein aggregates form. | ||
==Procedure on Atomic Absorption Spectrometry of Au/ | ==Procedure on Atomic Absorption Spectrometry of Au/lysozyme and Au/HRP solutions== | ||
* 8 of HCl samples with the concentrations below were run to set up calibration curve: | |||
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm | |||
* The absorbance obtained were graphed with absorption versus concentration of HCl. The slope of the plot was used to calculate the concentration of gold in Au/HRP and Au/lysozyme solutions. Because the absorption data and graph were not saved during experimental process, the results cannot be displayed in Data section. | |||
* Supernatant of Au/HRP samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/07|2012/11/07]] with a ratio ranging from 10 to 450 were run using Atomic Absorption Spectrometry. | |||
*Supernatant of Au/lysozyme samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/31|2012/10/31]] with a ratio ranging from 20 to 130 were also run using Atomic Absorption Spectrometry. | |||
==Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions== | ==Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions== |
Revision as of 15:01, 21 November 2012
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Purpose
Procedure on UV-vis spectrometry of Au/HRP
10 - 50 - 100 - 150 - 200 - 250 - 300 - 350 - 400 - 450
Data on UV-vis spectrometry of Au/HRP
Procedure on Atomic Absorption Spectrometry of Au/lysozyme and Au/HRP solutions
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions |