User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/07: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''HAuCl4 added [uL]''' | | align="center" style="background:#f0f0f0;"|'''HAuCl4 added [uL]''' | ||
| align="center" style="background:#f0f0f0;"|'''Water added [uL]''' | | align="center" style="background:#f0f0f0;"|'''Water added [uL]''' | ||
| align="center" style="background:#f0f0f0;"|'''Final concentration of Au[uM]''' | |||
| align="center" style="background:#f0f0f0;"|'''Final concentration of HRP[uM]''' | |||
|- | |- | ||
| 10||280.7||7.62||7711.68 | | 10||280.7||7.62||7711.68||1||10 | ||
|- | |- | ||
| 50||280.7||38.1||7681.2 | | 50||280.7||38.1||7681.2||1||50 | ||
|- | |- | ||
| 100||280.7||76.2||7643.1 | | 100||280.7||76.2||7643.1||1||100 | ||
|- | |- | ||
| 150||280.7||114.3||7605 | | 150||280.7||114.3||7605||1||150 | ||
|- | |- | ||
| 200||280.7||152.4||7566.9 | | 200||280.7||152.4||7566.9||1||200 | ||
|- | |- | ||
| 250||280.7||190.5||7528.8 | | 250||280.7||190.5||7528.8||1||250 | ||
|- | |- | ||
| 300||280.7||228.5||7490.8 | | 300||280.7||228.5||7490.8||1||300 | ||
|- | |- | ||
| 350||280.7||266.6||7452.7 | | 350||280.7||266.6||7452.7||1||350 | ||
|- | |- | ||
| 400||280.7||304.7||7414.6 | | 400||280.7||304.7||7414.6||1||400 | ||
|- | |- | ||
| 450||280.7||342.8||7376.5 | | 450||280.7||342.8||7376.5||1||450 | ||
|} | |} | ||
* Each Au/HRP samples were capped, wrapped with aluminum foil, and placed in incubator at 85°C for 4 hours then cooled down to room temperature. | * Each Au/HRP samples were capped, wrapped with aluminum foil, and placed in incubator at 85°C for 4 hours then cooled down to room temperature. |
Revision as of 21:05, 7 December 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure for Bradford Assay
Results and Calculations of ADA protein Concentrations
Absorbance = 27.609 * [BSA]mg/mL + 0.4363
Procedure for Making Au/HRP samples
10 - 50 - 100 - 150 - 200 - 250 - 300 - 350 - 400 - 450
28.5uM HRP * 0.005L = 1.5E-7 mol 1.5E-7mol * 40,000g/mol = 0.0057g of HRP
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