User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/04: Difference between revisions
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* Four aliquots of IPTG were added into each Fernbach flasks with cell culture before loaded into orbital shaker. | * Four aliquots of IPTG were added into each Fernbach flasks with cell culture before loaded into orbital shaker. | ||
* Cultures were left in orbital shaker for 4 hours, 210rpm, under 37°C. | * Cultures were left in orbital shaker for 4 hours, 210rpm, under 37°C. | ||
* After 4 hours of shaking, 1mL of cell were placed into a cuvette and run under UV-vis to test cell growth. The absorbance of cells in four Fernbach flasks were as follows: | * After 4 hours of shaking, 1mL of cell were placed into a cuvette and run under UV-vis to test cell growth. The UV-1800 UV-vis spectrometer from Shimadzu was used to measure absorbance via the following settings: | ||
Wavelength: 600nm | |||
Settings: Photometric | |||
Data display: Absorbance | |||
*The absorbance of cells in four Fernbach flasks were as follows: | |||
Revision as of 15:25, 4 November 2012
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Purpose
Procedure
Molecular weight: 238.31g/mol 0.4M × 0.004L = 0.0016mol of IPTG needed 238.31g/mol × 0.0016mol = 0.3813g of IPTG needed
Wavelength: 600nm Settings: Photometric Data display: Absorbance
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