User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/31 - Revision history

Keyun Wang: /* Purpose */

Keyun Wang — Sat, 08 Dec 2012 03:47:09 GMT

Purpose

←Older revision		Revision as of 03:47, 8 December 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Purpose==		==Purpose==
-	* Au/Lysozyme samples were made with the same procedure used to make Au/BSA solutions.	+	* Au/Lysozyme samples were made with the same procedure used to make [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17\|Au/BSA solutions on 2012/10/17]].

	==Procedure==		==Procedure==

Keyun Wang: /* Procedure */

Keyun Wang — Sat, 08 Dec 2012 03:44:32 GMT

Procedure

←Older revision		Revision as of 03:44, 8 December 2012
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	\| align="center" style="background:#f0f0f0;"\|'''HAuCl4 added [uL]'''		\| align="center" style="background:#f0f0f0;"\|'''HAuCl4 added [uL]'''
	\| align="center" style="background:#f0f0f0;"\|'''Water added [uL]'''		\| align="center" style="background:#f0f0f0;"\|'''Water added [uL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final Concentration of Au[mM]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final Concentration of Lysozyme[uM]'''
	\|-		\|-
-	\| 20\|\|114.3\|\|15.2\|\|7870.5	+	\| 20\|\|114.3\|\|15.2\|\|7870.5\|\|1\|\|20
	\|-		\|-
-	\| 30\|\|114.3\|\|22.9\|\|7862.8	+	\| 30\|\|114.3\|\|22.9\|\|7862.8\|\|1\|\|30
	\|-		\|-
-	\| 40\|\|114.3\|\|30.5\|\|7855.2	+	\| 40\|\|114.3\|\|30.5\|\|7855.2\|\|1\|\|40
	\|-		\|-
-	\| 50\|\|114.3\|\|38.1\|\|7847.6	+	\| 50\|\|114.3\|\|38.1\|\|7847.6\|\|1\|\|50
	\|-		\|-
-	\| 60\|\|114.3\|\|45.7\|\|7840	+	\| 60\|\|114.3\|\|45.7\|\|7840\|\|1\|\|60
	\|-		\|-
-	\| 70\|\|114.3\|\|53.3\|\|7832.4	+	\| 70\|\|114.3\|\|53.3\|\|7832.4\|\|1\|\|70
	\|-		\|-
-	\| 80\|\|114.3\|\|60.9\|\|7824.8	+	\| 80\|\|114.3\|\|60.9\|\|7824.8\|\|1\|\|80
	\|-		\|-
-	\| 100\|\|114.3\|\|76.2\|\|7809.5	+	\| 100\|\|114.3\|\|76.2\|\|7809.5\|\|1\|\|100
	\|-		\|-
-	\| 120\|\|114.3\|\|91.4\|\|7794.3	+	\| 120\|\|114.3\|\|91.4\|\|7794.3\|\|1\|\|120
	\|-		\|-
-	\| 130\|\|114.3\|\|99\|\|7786.7	+	\| 130\|\|114.3\|\|99\|\|7786.7\|\|1\|\|130
	\|}		\|}

Keyun Wang: /* Procedure */

Keyun Wang — Sat, 24 Nov 2012 17:01:25 GMT

Procedure

←Older revision		Revision as of 17:01, 24 November 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
-	* Lysozyme in powder form from chicken egg white obtained from ~~Signma~~ is used to make the lysozyme stock solution.	+	* Lysozyme in powder form from chicken egg white obtained from Signma® is used to make the lysozyme stock solution.
	* 5mL of 70μM lysozyme stock solution was made in the following way:		* 5mL of 70μM lysozyme stock solution was made in the following way:
	** 0.005g of lysozyme was weighted out		** 0.005g of lysozyme was weighted out

Keyun Wang: /* Procedure */

Keyun Wang — Sat, 24 Nov 2012 17:01:00 GMT

Procedure

←Older revision		Revision as of 17:01, 24 November 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
-	* Lysozyme in powder form obtained from ~~___company~~ is used to make the lysozyme stock solution.	+	* Lysozyme in powder form from chicken egg white obtained from Signma is used to make the lysozyme stock solution.
	* 5mL of 70μM lysozyme stock solution was made in the following way:		* 5mL of 70μM lysozyme stock solution was made in the following way:
	** 0.005g of lysozyme was weighted out		** 0.005g of lysozyme was weighted out

Keyun Wang at 04:23, 24 November 2012

Keyun Wang — Sat, 24 Nov 2012 04:23:46 GMT

←Older revision		Revision as of 04:23, 24 November 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here..~~.	+	* Au/Lysozyme samples were made with the same procedure used to make Au/BSA solutions.

		+	==Procedure==
		+	* Lysozyme in powder form obtained from ___company is used to make the lysozyme stock solution.
		+	* 5mL of 70μM lysozyme stock solution was made in the following way:
		+	** 0.005g of lysozyme was weighted out
		+	** 0.005g of lysozyme was transferred to a 15ml falcon tube and mixed well with 5mL of distilled water.
		+	** See [[User:Melissa Novy/Notebook/CHEM-571/2012/10/31\|Melissa's Notebook]] for calculation that stated that 0.005g of lysozyme was needed for 5mL of 70μM of lysozyme stock solution.
		+	* The HAuCl<sub>4</sub> used to make Au/Lysozyme samples was already made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]], and the concentration of the HAuCl<sub>4</sub> was 10mM.
		+	* Au/Lysozyme samples was made with mole ratios listed below:
		+	20 - 30 - 40 - 50 - 60 - 70 - 80 - 100 - 120 - 130
		+	* Based off the calculations for Au/BSA samples, a few changes were made to calculate the amount of lysozyme and HAuCl<sub>4</sub> needed for Au/Lysozyme samples.
		+	* The table below listed the initial conditions for both lysozyme and HAuCl<sub>4</sub> both theoretically and actually:
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''-'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Lysozyme'''
		+	\| align="center" style="background:#f0f0f0;"\|'''HAuCl<sub>4</sub>'''
		+	\|-
		+	\| Molecular Weight [g/mol]\|\|14300\|\|339.79
		+	\|-
		+	\| Theoretical Concentration [M]\|\|7.00E-05\|\|0.01
		+	\|-
		+	\| Volume of Water to add [L]\|\|0.005\|\|0.025
		+	\|-
		+	\| Moles Present [mol]\|\|3.50E-07\|\|0.00025
		+	\|-
		+	\| Theoretical Mass Needed [g]\|\|0.005\|\|0.0849
		+	\|-
		+	\| Actual Mass Weighted [g]\|\|0.005\|\|0.0892
		+	\|-
		+	\| Actual Concentration [M]\|\|0.0007\|\|10501
		+	\|}
		+
		+	* With the above calculation, the actual concentration of lysozyme came out to be 70μM and the actual concentration of HAuCl<sub>4</sub> came out to be 10.501mM. The two concentration was used to calculate the amount of water, lysozyme, HAuCl<sub>4</sub> needed to make the ratios listed above.
		+	* The table below listed the amount of water, lysozyme, HAuCl<sub>4</sub> used for each Au/Lysozyme sample:
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Au/Lysozyme ratio'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Lysozyme added [uL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''HAuCl4 added [uL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Water added [uL]'''
		+	\|-
		+	\| 20\|\|114.3\|\|15.2\|\|7870.5
		+	\|-
		+	\| 30\|\|114.3\|\|22.9\|\|7862.8
		+	\|-
		+	\| 40\|\|114.3\|\|30.5\|\|7855.2
		+	\|-
		+	\| 50\|\|114.3\|\|38.1\|\|7847.6
		+	\|-
		+	\| 60\|\|114.3\|\|45.7\|\|7840
		+	\|-
		+	\| 70\|\|114.3\|\|53.3\|\|7832.4
		+	\|-
		+	\| 80\|\|114.3\|\|60.9\|\|7824.8
		+	\|-
		+	\| 100\|\|114.3\|\|76.2\|\|7809.5
		+	\|-
		+	\| 120\|\|114.3\|\|91.4\|\|7794.3
		+	\|-
		+	\| 130\|\|114.3\|\|99\|\|7786.7
		+	\|}
		+
		+	* The stock solutions were added with pipetman and non-sterile pipette tips. All samples were made in 10mL large test tubes. All samples were capped and wrapped in aluminum foil.
		+	* All samples were placed in incubator at 85°C for four hours then in room temperature to cool down.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Keyun Wang: Autocreate 2012/10/31 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

Keyun Wang — Sat, 24 Nov 2012 03:44:40 GMT

Autocreate 2012/10/31 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__