User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/23 - Revision history

Keyun Wang at 20:05, 26 October 2012

Keyun Wang — Fri, 26 Oct 2012 20:05:47 GMT

←Older revision		Revision as of 20:05, 26 October 2012
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	0.004g X 66,000g/mol ÷ 0.001L = 9.2X10<sup>-6</sup>M = 9.2μM		0.004g X 66,000g/mol ÷ 0.001L = 9.2X10<sup>-6</sup>M = 9.2μM
	**Carbonate buffer		**Carbonate buffer
-	Carbonate buffer was made by mixing 100mL of 1M sodium bicarbonate with 100mL of 1M sodium bicarbonate together.	+	***Carbonate buffer was made by mixing 100mL of 1M sodium bicarbonate with 100mL of 1M sodium bicarbonate together.
-	pH of carbonate buffer was diluted by adding 1M and 6M HCl into the solution	+	***pH of carbonate buffer was diluted by adding 1M and 6M HCl into the solution
-	For calculations done to obtain the amount of grams weighted out the sodium carbonate and sodium bicarbonate, please consult [[User:Melissa Novy/Notebook/CHEM-571/2012/10/23\|Melissa's Notebook]].	+	***For calculations done to obtain the amount of grams weighted out the sodium carbonate and sodium bicarbonate, please consult [[User:Melissa Novy/Notebook/CHEM-571/2012/10/23\|Melissa's Notebook]].
-		+
	Molecular weight of sodium carbonate: 105.9784g/mol		Molecular weight of sodium carbonate: 105.9784g/mol
	0.0106g X 105.9784g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium carbonate		0.0106g X 105.9784g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium carbonate
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	Molecular weight of sodium bicarbonate: 84.007g/mol		Molecular weight of sodium bicarbonate: 84.007g/mol
	0.0084g X 84.007g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium bicarbonate		0.0084g X 84.007g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium bicarbonate
-		+	***The 100mL of 1M sodium bicarbonate is mixed with 100mL of 1M sodium carbonate in a 1000mL graduated cylinder. pH was taken with a pH meter and the pH turned out to be 10.5.
-	The 100mL of 1M sodium bicarbonate is mixed with 100mL of 1M sodium carbonate in a 1000mL graduated cylinder. pH was taken with a pH meter and the pH turned out to be 10.5.	+	***Initially 1M HCl was added drop wise into the carbonate buffer mix. About 50mL of 1M HCl was added to carbonate buffer mix to adjust the pH to 9.5. Then, 6M HCl was added to speed up pH adjustment process. About 10mL of 6N HCl was added drop wise to adjust the pH to 8.5.
-	Initially 1M HCl was added drop wise into the carbonate buffer mix. About 50mL of 1M HCl was added to carbonate buffer mix to adjust the pH to 9.5. Then, 6M HCl was added to speed up pH adjustment process. About 10mL of 6N HCl was added drop wise to adjust the pH to 8.5.	+	***Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/10/23\|Melissa's Notebook]] for calculations on determining the new molar concentration of carbonate buffer. The final concentration of carbonate buffer is calculated to be 0.833mM. Thus, 256mL of 0.833mM carbonate was made for luminol assay the next day.
-	Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/10/23\|Melissa's Notebook]] for calculations on determining the new molar concentration of carbonate buffer. The final concentration of carbonate buffer is calculated to be 0.833mM. Thus, 256mL of 0.833mM carbonate was made for luminol assay the next day.	+

Keyun Wang at 20:03, 26 October 2012

Keyun Wang — Fri, 26 Oct 2012 20:03:49 GMT

←Older revision		Revision as of 20:03, 26 October 2012
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	0.0084g X 84.007g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium bicarbonate		0.0084g X 84.007g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium bicarbonate

-		+	The 100mL of 1M sodium bicarbonate is mixed with 100mL of 1M sodium carbonate in a 1000mL graduated cylinder. pH was taken with a pH meter and the pH turned out to be 10.5.
		+	Initially 1M HCl was added drop wise into the carbonate buffer mix. About 50mL of 1M HCl was added to carbonate buffer mix to adjust the pH to 9.5. Then, 6M HCl was added to speed up pH adjustment process. About 10mL of 6N HCl was added drop wise to adjust the pH to 8.5.
		+	Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/10/23\|Melissa's Notebook]] for calculations on determining the new molar concentration of carbonate buffer. The final concentration of carbonate buffer is calculated to be 0.833mM. Thus, 256mL of 0.833mM carbonate was made for luminol assay the next day.

Keyun Wang at 16:25, 26 October 2012

Keyun Wang — Fri, 26 Oct 2012 16:25:28 GMT

←Older revision		Revision as of 16:25, 26 October 2012
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	==Purpose==		==Purpose==
	* Clean up and make room for samples of Lysozyme/Gold solutions		* Clean up and make room for samples of Lysozyme/Gold solutions
-	* Make ~~gold/lysozyme~~ solutions ~~in with the following ratios~~:	+	* Make stock solutions for Luminol assay experiment tomorrow: Luminol, Horseradish peroxidase, H2O2, sodium carbonate buffer.
-	~~10-15-20-25-30-35-40~~	+

	==Procedure==		==Procedure==
		+	* Test tubes and falcon tubes were washed for clean up.
		+	* A serious of stocks solutions were made for testing luminol experiment the next day.
		+	**Phenol: concentration of 18mM 4-Iodophenol was not made because about 18mM phenol stock solution pre-made from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/18\|2012/09/18]] (check this data entry for calculations made and actual concentration of phenol) is going to be used for luminol assay due to its long shelf life in room temperature.
		+	**Horseradish peroxidase
		+	Horseradish peroxidase was made by weighting out 0.004g of horseradish peroxidase power.
		+	The weighted out amount was mixed with 1mL of sterile water.
		+	Molecular weight of horseradish peroxidase is 66kDa.
		+	Calculation is depicted as follows:
		+	0.004g X 66,000g/mol ÷ 0.001L = 9.2X10<sup>-6</sup>M = 9.2μM
		+	**Carbonate buffer
		+	Carbonate buffer was made by mixing 100mL of 1M sodium bicarbonate with 100mL of 1M sodium bicarbonate together.
		+	pH of carbonate buffer was diluted by adding 1M and 6M HCl into the solution
		+	For calculations done to obtain the amount of grams weighted out the sodium carbonate and sodium bicarbonate, please consult [[User:Melissa Novy/Notebook/CHEM-571/2012/10/23\|Melissa's Notebook]].

		+	Molecular weight of sodium carbonate: 105.9784g/mol
		+	0.0106g X 105.9784g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium carbonate
		+
		+	Molecular weight of sodium bicarbonate: 84.007g/mol
		+	0.0084g X 84.007g/mol ÷ 0.1L = 1X10<sup>-3</sup>M = 1mM sodium bicarbonate
		+
		+

Keyun Wang at 19:22, 23 October 2012

Keyun Wang — Tue, 23 Oct 2012 19:22:03 GMT

←Older revision		Revision as of 19:22, 23 October 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here...~~	+	* Clean up and make room for samples of Lysozyme/Gold solutions
		+	* Make gold/lysozyme solutions in with the following ratios:
		+	10-15-20-25-30-35-40
		+
		+	==Procedure==
		+

Keyun Wang: Autocreate 2012/10/23 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

Keyun Wang — Tue, 23 Oct 2012 16:21:30 GMT

Autocreate 2012/10/23 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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