User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17: Difference between revisions
From OpenWetWare
Keyun Wang (talk | contribs) No edit summary |
Keyun Wang (talk | contribs) No edit summary |
||
Line 35: | Line 35: | ||
==Results== | ==Results== | ||
* | * Gel were observed under UV. | ||
* The atomic absorbance spectrometer were run on samples, tables is shown below for standard calibration and concentration of gold measured. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''standard''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''background''' | |||
|- | |||
| blank||||-0.1347||0.0015 | |||
|- | |||
| 5||-||0.1152||0.002 | |||
|- | |||
| 8||-||0.1795||0.0057 | |||
|- | |||
| 10||-||0.2326||0.0098 | |||
|- | |||
| 15||-||0.3279||0.0085 | |||
|- | |||
| 20||-||0.4342||0.0092 | |||
|- | |||
| 25||-||0.5281||0.0103 | |||
|- | |||
| 30||-||0.6326||0.0125 | |||
|- | |||
| 40||-||0.7752||0.0126 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''background''' | |||
|- | |||
| 80||32.3627||0.6573||0.0154 | |||
|- | |||
| 100||33.5087||0.6793||0.0112 | |||
|- | |||
| 120||32.1596||0.6534||0.0137 | |||
|- | |||
| 128||23.4034||0.4853||0.0145 | |||
|- | |||
| 130||22.096||0.4602||0.0138 | |||
|- | |||
| 132||32.0606||0.6515||0.0157 | |||
|- | |||
| 133||8.9596||0.208||0.0128 | |||
|- | |||
| 134||-1.4429||0.0083||0.0091 | |||
|- | |||
| 136||-0.198||0.0322||0.0075 | |||
|- | |||
| 138||-1.7398||0.0026||0.006 | |||
|- | |||
| 160||-2.0315||-0.003||0.0101 | |||
|- | |||
| 170||-1.469||0.0078||0.0067 | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 12:47, 17 October 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
DNA ladder --> E34K --> E34A --> E34A --> K110A --> K110A --> E34K
2000rpm for 5 minutes, 2000rpm for 10 minutes, 2500rpm for 5 minutes, 3000rpm for 10 minutes, 4700rpm for 15 minutes, 4700rpm for 20 minutes
5ppm, 8ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 40ppm
Notes
Results
|