User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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4700rpm for 15 minutes, 4700rpm for 20 minutes | 4700rpm for 15 minutes, 4700rpm for 20 minutes | ||
* Some fibers in some solutions still remained on top of solution. The supernatant were taken out using a pipet and placed in 15mL falcon tube. | * Some fibers in some solutions still remained on top of solution. The supernatant were taken out using a pipet and placed in 15mL falcon tube. | ||
* Supernatant with different ratios of Au/BSA were run under atomic absorbance spectrophotometer. | * Supernatant with different ratios of Au/BSA were run under atomic absorbance spectrophotometer. Blank was done with HCl. Wavelength is excited at 242.8nm. 8 standards were run at the following concentration: | ||
5ppm, 8ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 40ppm | |||
* 12 samples of Au/BSA with various ratios were run in atomic absorbance spectrophotometer after standards were taken. | |||
==Notes== | ==Notes== |
Revision as of 12:26, 17 October 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Purpose
Procedure
DNA ladder --> E34K --> E34A --> E34A --> K110A --> K110A --> E34K
2000rpm for 5 minutes, 2000rpm for 10 minutes, 2500rpm for 5 minutes, 3000rpm for 10 minutes, 4700rpm for 15 minutes, 4700rpm for 20 minutes
5ppm, 8ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 40ppm
Notes
Results |