User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/02

Purpose

• Extract protein from cells as part of protein purification.

Procedure

• Cells were taken out of -80C freezer and placed in ~30C warm water for cell thawing.
• Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.
• Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
• Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.

Discussion