User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/02 - Revision history

Keyun Wang at 03:35, 27 October 2012

Keyun Wang — Sat, 27 Oct 2012 03:35:17 GMT

←Older revision		Revision as of 03:35, 27 October 2012
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	* Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.		* Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.
	* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.		* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
-	* Once centrifuged, supernatant were kept, filtered with ~~450mm~~ filter paper, and kept in 4C until the next day.	+	* Once centrifuged, supernatant were kept, filtered with 450nm filter paper, and kept in 4C until the next day.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:53, 7 October 2012 (EDT)''':it is are you sure it is 450 mm? tat is .45 m pores? seems very large?	+
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:54, 7 October 2012 (EDT)''': is this all you did? did you filter the buffers? make the buffers?	+

	==Discussion==		==Discussion==

Abigail E. Miller: /* Procedure */

Abigail E. Miller — Sun, 07 Oct 2012 21:54:12 GMT

Procedure

←Older revision		Revision as of 21:54, 7 October 2012
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	* Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.		* Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.
	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:53, 7 October 2012 (EDT)''':it is are you sure it is 450 mm? tat is .45 m pores? seems very large?		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:53, 7 October 2012 (EDT)''':it is are you sure it is 450 mm? tat is .45 m pores? seems very large?
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:54, 7 October 2012 (EDT)''': is this all you did? did you filter the buffers? make the buffers?

	==Discussion==		==Discussion==

Abigail E. Miller: /* Procedure */

Abigail E. Miller — Sun, 07 Oct 2012 21:53:03 GMT

Procedure

←Older revision		Revision as of 21:53, 7 October 2012
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	* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.		* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
	* Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.		* Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:53, 7 October 2012 (EDT)''':it is are you sure it is 450 mm? tat is .45 m pores? seems very large?

	==Discussion==		==Discussion==

Keyun Wang: /* Discussion */

Keyun Wang — Wed, 03 Oct 2012 19:07:23 GMT

Discussion

←Older revision		Revision as of 19:07, 3 October 2012
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	==Discussion==		==Discussion==
-		+	* Cells taken from the freezer were complete cells. After sonication, cells were burst open. The organelles, plasma membrane, and internal proteins were all exposed in solution. Centrifugation allowed the heavier organelles and plasma membranes to sink to the bottom of the tube, forming a pellet, and remaining proteins or other lighter biological molecules in supernatant. This is an effective and simple way to purify proteins from prokaryotic cells.

Keyun Wang at 19:04, 3 October 2012

Keyun Wang — Wed, 03 Oct 2012 19:04:45 GMT

←Older revision		Revision as of 19:04, 3 October 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here~~...	+	* Extract protein from cells as part of protein purification.
		+
		+	==Procedure==
		+	* Cells were taken out of -80C freezer and placed in ~30C warm water for cell thawing.
		+	* Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.
		+	* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
		+	* Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.
		+
		+	==Discussion==
		+

Keyun Wang: Autocreate 2012/10/02 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

Keyun Wang — Wed, 03 Oct 2012 19:00:55 GMT

Autocreate 2012/10/02 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


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