User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/02: Difference between revisions
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* Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times. | * Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times. | ||
* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C. | * Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C. | ||
* Once centrifuged, supernatant were kept, filtered with | * Once centrifuged, supernatant were kept, filtered with 450nm filter paper, and kept in 4C until the next day. | ||
==Discussion== | ==Discussion== |
Revision as of 20:35, 26 October 2012
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Purpose
Procedure
Discussion
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