User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/26 - Revision history

Keyun Wang: /* Procedure */

Keyun Wang — Sat, 27 Oct 2012 03:34:45 GMT

Procedure

←Older revision		Revision as of 03:34, 27 October 2012
Line 15:		Line 15:
	* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.		* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.
	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells.		* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells.
-	* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the ~~experience~~, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table:	+	* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experiment, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table:
	{\| {{table}}		{\| {{table}}
	\| align="center" style="background:#f0f0f0;"\|''''''		\| align="center" style="background:#f0f0f0;"\|''''''

Keyun Wang at 03:34, 27 October 2012

Keyun Wang — Sat, 27 Oct 2012 03:34:19 GMT

←Older revision		Revision as of 03:34, 27 October 2012
Line 14:		Line 14:
	* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.		* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.
	* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.		* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':you need to include the actual chemical composition and concentrations and pH of hte buffers.	+	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells.
-	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week.	+	* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table:
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':didn't this require two ~~runs of the centrifuge due to volume? that should be noted here~~.	+	{\| {{table}}
-	* Luminol-peroxide reaction were further ~~experienced~~. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial.	+	\| align="center" style="background:#f0f0f0;"\|''''''
-	*'''[~~[User~~:~~Abigail E. Miller~~\|~~Abigail E. Miller~~]~~] 17~~:~~51, 7 October 2012 (EDT)~~''':experience or experiment? you need to include more information and all the data ie enough information to explain the data presented. what was varied? and why? details of how those concentratiosn were varied can be found in Melissa'~~s notebook~~.	+	\| align="center" style="background:#f0f0f0;"\|'''Initial Concentrations [mM]'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final Concentrations [mM]'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\|-
		+	\| Trial\|\|4-Iodophenol\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|HRP\|\|4-Iodophenol\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|HRP
		+	\|-
		+	\| 1\|\|18\|\|1.25\|\|1.7\|\|0.0023\|\|0.000119\|\|0.000578\|\|0.000846\|\|7.63E-08
		+	\|-
		+	\| 2\|\|18\|\|0.625\|\|1.7\|\|0.0023\|\|0.000119\|\|0.000289\|\|0.000846\|\|7.63E-08
		+	\|-
		+	\| 3\|\|18\|\|0.625\|\|0.85\|\|0.0023\|\|0.000119\|\|0.000289\|\|0.000423\|\|7.63E-08
		+	\|-
		+	\| 4\|\|18\|\|1.25\|\|0.425\|\|0.0023\|\|0.000119\|\|0.000578\|\|0.000212\|\|7.63E-08
		+	\|-
		+	\| 5\|\|18\|\|1.25\|\|1.7\|\|0.0023\|\|0.000119\|\|0.000578\|\|0.000846\|\|7.63E-08
		+	\|-
		+	\| 6\|\|18\|\|1.25\|\|1.7\|\|0.00115\|\|0.000119\|\|0.000578\|\|0.000846\|\|3.82E-08
		+	\|}

	==Notes==		==Notes==

Abigail E. Miller: /* Procedure */

Abigail E. Miller — Sun, 07 Oct 2012 21:51:32 GMT

Procedure

←Older revision		Revision as of 21:51, 7 October 2012
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	* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.		* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.
	* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.		* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':you need to include the actual chemical composition and concentrations and pH of hte buffers.
	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week.		* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':didn't this require two runs of the centrifuge due to volume? that should be noted here.
	* Luminol-peroxide reaction were further experienced. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial.		* Luminol-peroxide reaction were further experienced. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':experience or experiment? you need to include more information and all the data ie enough information to explain the data presented. what was varied? and why? details of how those concentratiosn were varied can be found in Melissa's notebook.

	==Notes==		==Notes==

Keyun Wang at 05:12, 5 October 2012

Keyun Wang — Fri, 05 Oct 2012 05:12:03 GMT

←Older revision		Revision as of 05:12, 5 October 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here~~...	+	* To continue cell amplification from yesterday for greater yield of ADA proteins
		+	* To continue test out the best concentration of reactants for luminol-peroxide reaction in addition to horseradish peroxidase
		+
		+	==Procedure==
		+	* 0.4M IPTG for protein expression were made by mixing 0.4g of IPTG with 1mL of sterile water.
		+	* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.
		+	* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.
		+	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week.
		+	* Luminol-peroxide reaction were further experienced. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial.
		+
		+	==Notes==
		+	* Cells were amplified by transferring from 4mL LB broth to 250mL LB broth, then to 1L LB broth. This sequence of steps created ideal environment for cells to divide at fastest rate.
		+	* Cells were centrifuged in order to seperate the cells from LB broth before transferring into new broth. That way cells placed in new LB broth were the most concentrated.
		+	* Binding and elution buffers will later be used in protein purification. The imidazol concentration in both buffers were directed towards the purification of adenosine deaminase protein.
		+	* It was still not concluded the concentration of luminol and peroxide concentration through experimenting the concentration reactants and enzyme. However, it was concluded that the increase of enzyme concentration increases reaction speed. It was suggested turning the lights off in lab room while injecting horse radish peroxide with the fluorometer running in order for fluorometer to catch the quick luminescence reaction. For details of experimented concentration of reactants and enzymes, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]].
		+

Keyun Wang: Autocreate 2012/09/26 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

Keyun Wang — Thu, 04 Oct 2012 14:32:48 GMT

Autocreate 2012/09/26 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__

←Older revision		Revision as of 03:34, 27 October 2012
Line 15:		Line 15:
	* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.		* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.
	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells.		* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells.
-	* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the ~~experience~~, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table:	+	* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experiment, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table:
	{\| {{table}}		{\| {{table}}
	\| align="center" style="background:#f0f0f0;"\|''''''		\| align="center" style="background:#f0f0f0;"\|''''''

←Older revision		Revision as of 03:34, 27 October 2012
Line 14:		Line 14:
	* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.		* In the morning, cells left in shaker were taken out and spin in centrifuge at 4500rpm for 15 minutes. Pellets form, and supernatant were discarded. Pellet were resuspended in 4mL of LB. 1mL of each cell culture were placed in 1L autoclaved sterile LB broth, 1mL of 0.4M IPTG were added before incubation. Cell culture were placed in shaker under 225rpm at 37C for ~4 hours.
	* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.		* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26\|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':you need to include the actual chemical composition and concentrations and pH of hte buffers.	+	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells.
-	* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week.	+	* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table:
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:51, 7 October 2012 (EDT)''':didn't this require two ~~runs of the centrifuge due to volume? that should be noted here~~.	+	{\| {{table}}
-	* Luminol-peroxide reaction were further ~~experienced~~. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experience, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26\|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial.	+	\| align="center" style="background:#f0f0f0;"\|''''''
-	*'''[~~[User~~:~~Abigail E. Miller~~\|~~Abigail E. Miller~~]~~] 17~~:~~51, 7 October 2012 (EDT)~~''':experience or experiment? you need to include more information and all the data ie enough information to explain the data presented. what was varied? and why? details of how those concentratiosn were varied can be found in Melissa'~~s notebook~~.	+	\| align="center" style="background:#f0f0f0;"\|'''Initial Concentrations [mM]'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final Concentrations [mM]'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\|-
		+	\| Trial\|\|4-Iodophenol\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|HRP\|\|4-Iodophenol\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|HRP
		+	\|-
		+	\| 1\|\|18\|\|1.25\|\|1.7\|\|0.0023\|\|0.000119\|\|0.000578\|\|0.000846\|\|7.63E-08
		+	\|-
		+	\| 2\|\|18\|\|0.625\|\|1.7\|\|0.0023\|\|0.000119\|\|0.000289\|\|0.000846\|\|7.63E-08
		+	\|-
		+	\| 3\|\|18\|\|0.625\|\|0.85\|\|0.0023\|\|0.000119\|\|0.000289\|\|0.000423\|\|7.63E-08
		+	\|-
		+	\| 4\|\|18\|\|1.25\|\|0.425\|\|0.0023\|\|0.000119\|\|0.000578\|\|0.000212\|\|7.63E-08
		+	\|-
		+	\| 5\|\|18\|\|1.25\|\|1.7\|\|0.0023\|\|0.000119\|\|0.000578\|\|0.000846\|\|7.63E-08
		+	\|-
		+	\| 6\|\|18\|\|1.25\|\|1.7\|\|0.00115\|\|0.000119\|\|0.000578\|\|0.000846\|\|3.82E-08
		+	\|}

	==Notes==		==Notes==