User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/26: Difference between revisions
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* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification. | * Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification. | ||
* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells. | * After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells. | ||
* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the | * Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experiment, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table: | ||
{| {{table}} | {| {{table}} | ||
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Revision as of 20:34, 26 October 2012
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Purpose
Procedure
Notes
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