User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/25: Difference between revisions

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(Autocreate 2012/09/25 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* To start culture for cell growth with transformed ADA strand cells
* Continue with HRP assay measurements using florometer
 
==Procedure==
* Four LB solution for starter culture was made by mixing 0.875g of LB in 35mL of distilled water in 250mL Erlenmeyer flasks. Four of 1L LB solution for cell amplification 250g with 1L distilled water in 2.8L Erlenmeyer flasks. Broth were autoclaved in liquid cycle.
* New horseradish peroxidase were made by mixing 1mg of horseradish peroxidase in 1mL of distilled water. The calculated final concentration of horseradish peroxidase is 2.3uM. The enzyme were diluted ten folds to 230nM and 23nM to test luminol and H2O2 reaction.
* New 4-Iodophenol was made with water and sodium bicarbonate. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/25|Melissa's Notebook]] for details on making the 4-Iodophenol stock solution.
 
==Notes==
 




Revision as of 10:52, 25 September 2012

Experimental Biological Chemistry I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • To start culture for cell growth with transformed ADA strand cells
  • Continue with HRP assay measurements using florometer

Procedure

  • Four LB solution for starter culture was made by mixing 0.875g of LB in 35mL of distilled water in 250mL Erlenmeyer flasks. Four of 1L LB solution for cell amplification 250g with 1L distilled water in 2.8L Erlenmeyer flasks. Broth were autoclaved in liquid cycle.
  • New horseradish peroxidase were made by mixing 1mg of horseradish peroxidase in 1mL of distilled water. The calculated final concentration of horseradish peroxidase is 2.3uM. The enzyme were diluted ten folds to 230nM and 23nM to test luminol and H2O2 reaction.
  • New 4-Iodophenol was made with water and sodium bicarbonate. See Melissa's Notebook for details on making the 4-Iodophenol stock solution.

Notes


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