User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/19: Difference between revisions
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==Procedure== | ==Procedure== | ||
* Stock solutions were pre-made 24 hours ago before samples were mixed and run under fluorometer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18|Melissa's Notebook]] for details in making stock solutions on concentration of horseradish peroxidase and 4-Idophenol that's used in solutions. | * Stock solutions were pre-made 24 hours ago before samples were mixed and run under fluorometer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18|Melissa's Notebook]] for details in making stock solutions on concentration of horseradish peroxidase and 4-Idophenol that's used in solutions. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 17:41, 7 October 2012 (EDT)''':again be specific on what are the stock concentrations. | |||
* Luminol stock solution was diluted to 2.5mM and mixed with carbonate buffer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/19|Melissa's Notebook]] for details of the dilution and reasoning behind making the luminol stock. | * Luminol stock solution was diluted to 2.5mM and mixed with carbonate buffer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/19|Melissa's Notebook]] for details of the dilution and reasoning behind making the luminol stock. | ||
* First sample was mixed with 4-Idophenol, H2O2, luminol, and horseradish peroxidase with the concentration below, respectively, before measurements were taken. For details in concentration of stocks added, see below table: | * First sample was mixed with 4-Idophenol, H2O2, luminol, and horseradish peroxidase with the concentration below, respectively, before measurements were taken. For details in concentration of stocks added, see below table: |
Revision as of 14:41, 7 October 2012
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Purpose
Procedure
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