User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/19: Difference between revisions
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* Samples with varying concentrations of luminol or H2O2 were diluted from those stock solutions. To make 625uM luminol, 50uL of 1.25uM was mixed with 50uL of distilled water. To make 850uM of H2O2, 50uL of 1.7mM H2O2 was mixed with 50uL of distilled water. | * Samples with varying concentrations of luminol or H2O2 were diluted from those stock solutions. To make 625uM luminol, 50uL of 1.25uM was mixed with 50uL of distilled water. To make 850uM of H2O2, 50uL of 1.7mM H2O2 was mixed with 50uL of distilled water. | ||
* Fluorometer was set to wavelength 430nm. All data were collected for a period of ~300 seconds to make sure samples has reached equilibrium and had completed in fluorescing. | * Fluorometer was set to wavelength 430nm. All data were collected for a period of ~300 seconds to make sure samples has reached equilibrium and had completed in fluorescing. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 17:42, 7 October 2012 (EDT)''':what volumes of each concentrations were added to achieve the final volumes? | |||
==Notes== | ==Notes== |
Revision as of 14:42, 7 October 2012
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Purpose
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