User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/19: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* To determine enzymatic activity of horseradish peroxidase via the usage of fluorometer under 510nm.


==Procedure==
* Stock solutions were pre-made 24 hours ago before samples were mixed and run under fluorometer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18|Melissa's Notebook]] for details in making stock solutions on concentration of horseradish peroxidase and 4-Idophenol that's used in solutions.
* Luminol stock solution was diluted to 2.5mM and mixed with carbonate buffer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/19|Melissa's Notebook]] for details of the dilution and reasoning behind making the luminol stock.
* Samples were mixed with 4-Idophenol, H2O2, luminol, and horseradish peroxidase, respectively, before measurements were taken. For details in concentration of stocks added, see below table:
* Fluorometer was set to wavelength 510nm. All data were collected for a period of ~300 seconds to make sure samples has reached equilibrium and had completed in fluorescing.
==Notes==
* Fluorescence at 510nm was plotted against time in seconds. Only three samples were run: 1.25mM luminol and 1.7mM H2O2, 625uM luminol and 1.7mM H2O2, and 625uM luminol and 850uM H2O2. Within the samples also added 18mM 4-iodophenol and 2.3uM horseradish peroxidase.
[[Image:Fluorescence at 510nm versus time-s-.png|800px]]


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Revision as of 21:57, 24 September 2012

Experimental Biological Chemistry I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • To determine enzymatic activity of horseradish peroxidase via the usage of fluorometer under 510nm.

Procedure

  • Stock solutions were pre-made 24 hours ago before samples were mixed and run under fluorometer. See Melissa's Notebook for details in making stock solutions on concentration of horseradish peroxidase and 4-Idophenol that's used in solutions.
  • Luminol stock solution was diluted to 2.5mM and mixed with carbonate buffer. See Melissa's Notebook for details of the dilution and reasoning behind making the luminol stock.
  • Samples were mixed with 4-Idophenol, H2O2, luminol, and horseradish peroxidase, respectively, before measurements were taken. For details in concentration of stocks added, see below table:
  • Fluorometer was set to wavelength 510nm. All data were collected for a period of ~300 seconds to make sure samples has reached equilibrium and had completed in fluorescing.

Notes

  • Fluorescence at 510nm was plotted against time in seconds. Only three samples were run: 1.25mM luminol and 1.7mM H2O2, 625uM luminol and 1.7mM H2O2, and 625uM luminol and 850uM H2O2. Within the samples also added 18mM 4-iodophenol and 2.3uM horseradish peroxidase.


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