User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/19
From OpenWetWare
(Difference between revisions)
(Autocreate 2012/09/19 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I) |
|||
| Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
| - | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | + | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> |
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
| - | == | + | ==Purpose== |
| - | * | + | * To determine enzymatic activity of horseradish peroxidase via the usage of fluorometer under 510nm. |
| + | ==Procedure== | ||
| + | * Stock solutions were pre-made 24 hours ago before samples were mixed and run under fluorometer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18|Melissa's Notebook]] for details in making stock solutions on concentration of horseradish peroxidase and 4-Idophenol that's used in solutions. | ||
| + | * Luminol stock solution was diluted to 2.5mM and mixed with carbonate buffer. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/19|Melissa's Notebook]] for details of the dilution and reasoning behind making the luminol stock. | ||
| + | * Samples were mixed with 4-Idophenol, H2O2, luminol, and horseradish peroxidase, respectively, before measurements were taken. For details in concentration of stocks added, see below table: | ||
| + | |||
| + | * Fluorometer was set to wavelength 510nm. All data were collected for a period of ~300 seconds to make sure samples has reached equilibrium and had completed in fluorescing. | ||
| + | |||
| + | ==Notes== | ||
| + | * Fluorescence at 510nm was plotted against time in seconds. Only three samples were run: 1.25mM luminol and 1.7mM H2O2, 625uM luminol and 1.7mM H2O2, and 625uM luminol and 850uM H2O2. Within the samples also added 18mM 4-iodophenol and 2.3uM horseradish peroxidase. | ||
| + | [[Image:Fluorescence at 510nm versus time-s-.png|800px]] | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 00:57, 25 September 2012
 Experimental Biological Chemistry I
|  Main project page Previous entry Next entry
|
Purpose
Procedure
Notes
| |
