User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/18 - Revision history

Keyun Wang: /* Notes */

2012-10-27T03:21:26Z

Notes

←Older revision		Revision as of 03:21, 27 October 2012
Line 32:		Line 32:
	* It was discovered that when concentration of AAP is held constant, the enzymatic activity decreases once the concentration of H2O2 increases. This could be concluded by comparing the slope of each sample with different concentrations of H2O2.		* It was discovered that when concentration of AAP is held constant, the enzymatic activity decreases once the concentration of H2O2 increases. This could be concluded by comparing the slope of each sample with different concentrations of H2O2.
	* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.		* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.
		+	* Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for the two graphs: Absorbance over Time of Enzymatic Activity of HRP in fixed [AAP] and Varied [H2O2], and Absorbance over Time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP].

Keyun Wang at 03:19, 27 October 2012

2012-10-27T03:19:05Z

←Older revision		Revision as of 03:19, 27 October 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
-	* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for the final concentration of stock solution for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.	+	* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for the final concentration of stock solution and final concentration reached for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:29, 7 October 2012 (EDT)''':you need to list the final concentrations reached	+
	* Samples mixed with altering concentration of H2O2 was made and analyzed under UV-vis spectrometer. The concentration of AAP(4-aminoantipyrine) was set at 2.5mM, 4-Iodophenol was set at 18mM, and horseradish peroxidase at 2.3uM. The concentration of H2O2 was changed from 1.7mM to 106.25uM.		* Samples mixed with altering concentration of H2O2 was made and analyzed under UV-vis spectrometer. The concentration of AAP(4-aminoantipyrine) was set at 2.5mM, 4-Iodophenol was set at 18mM, and horseradish peroxidase at 2.3uM. The concentration of H2O2 was changed from 1.7mM to 106.25uM.
	* In the next set of samples, concentration of H2O2 is held constant. The concentration of AAP was altered from 1.25mM to 156.25uM. The concentration of other solutions were kept the same.		* In the next set of samples, concentration of H2O2 is held constant. The concentration of AAP was altered from 1.25mM to 156.25uM. The concentration of other solutions were kept the same.
-	* For results obtained from UV-vis spectrometer, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for graph of Absorbance versus Time of enzymatic activity of HRP in Fixed [AAP] and varied [H2O2], and in Fixed [H2O2] and varied [AAP]	+	* For results obtained from UV-vis spectrometer, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for graph of Absorbance versus Time of enzymatic activity of HRP in Fixed [AAP] and varied [H2O2], and in Fixed [H2O2] and varied [AAP]. In those samples, the initial and final concentration reached were depicted in the table below:
-		+	{\| {{table}}
-	*'''[~~[User~~:~~Abigail E. Miller~~\|~~Abigail E. Miller~~]~~] 17~~:29, ~~7 October 2012 (EDT)~~'''~~:how did you reach all these concnetrations? what volumes were used? be specific~~. ~~what concentrations specifically were tested and why? how are they labeled? how do i tell which is which? results shoudl be in your notebook, not referenced~~.	+	\| align="center" style="background:#f0f0f0;"\|'''Stock Solution'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Initial Concentration, M<sub>1</sub> [M]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume Added V<sub>1</sub> [mL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final Concentration, M<sub>2</sub> [M]'''
		+	\|-
		+	\| H<sub>2</sub>O<sub>2</sub>\|\|0.000034\|\|0.75\|\|0.000017
		+	\|-
		+	\| AAP\|\|0.0025\|\|0.7\|\|0.001166667
		+	\|-
		+	\| Phenol\|\|0.018\|\|0.01\|\|0.00012
		+	\|-
		+	\|}

	==Notes==		==Notes==
Line 23:		Line 33:
	* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.		* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.

-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:29, 7 October 2012 (EDT)''':you ne3ed ot include the graphs. and the rates otherwise there is no evidence to back your conclusions.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Abigail E. Miller at 21:29, 7 October 2012

2012-10-07T21:29:40Z

←Older revision		Revision as of 21:29, 7 October 2012
Line 11:		Line 11:
	==Procedure==		==Procedure==
	* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for the final concentration of stock solution for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.		* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for the final concentration of stock solution for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:29, 7 October 2012 (EDT)''':you need to list the final concentrations reached
	* Samples mixed with altering concentration of H2O2 was made and analyzed under UV-vis spectrometer. The concentration of AAP(4-aminoantipyrine) was set at 2.5mM, 4-Iodophenol was set at 18mM, and horseradish peroxidase at 2.3uM. The concentration of H2O2 was changed from 1.7mM to 106.25uM.		* Samples mixed with altering concentration of H2O2 was made and analyzed under UV-vis spectrometer. The concentration of AAP(4-aminoantipyrine) was set at 2.5mM, 4-Iodophenol was set at 18mM, and horseradish peroxidase at 2.3uM. The concentration of H2O2 was changed from 1.7mM to 106.25uM.
	* In the next set of samples, concentration of H2O2 is held constant. The concentration of AAP was altered from 1.25mM to 156.25uM. The concentration of other solutions were kept the same.		* In the next set of samples, concentration of H2O2 is held constant. The concentration of AAP was altered from 1.25mM to 156.25uM. The concentration of other solutions were kept the same.
	* For results obtained from UV-vis spectrometer, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for graph of Absorbance versus Time of enzymatic activity of HRP in Fixed [AAP] and varied [H2O2], and in Fixed [H2O2] and varied [AAP]		* For results obtained from UV-vis spectrometer, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for graph of Absorbance versus Time of enzymatic activity of HRP in Fixed [AAP] and varied [H2O2], and in Fixed [H2O2] and varied [AAP]
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:29, 7 October 2012 (EDT)''':how did you reach all these concnetrations? what volumes were used? be specific. what concentrations specifically were tested and why? how are they labeled? how do i tell which is which? results shoudl be in your notebook, not referenced.

	==Notes==		==Notes==
Line 19:		Line 22:
	* It was discovered that when concentration of AAP is held constant, the enzymatic activity decreases once the concentration of H2O2 increases. This could be concluded by comparing the slope of each sample with different concentrations of H2O2.		* It was discovered that when concentration of AAP is held constant, the enzymatic activity decreases once the concentration of H2O2 increases. This could be concluded by comparing the slope of each sample with different concentrations of H2O2.
	* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.		* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 17:29, 7 October 2012 (EDT)''':you ne3ed ot include the graphs. and the rates otherwise there is no evidence to back your conclusions.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Keyun Wang: /* Purpose */

2012-09-25T04:11:57Z

Purpose

←Older revision		Revision as of 04:11, 25 September 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Purpose==		==Purpose==
-	* To make Horseradish Peroxidase Assay in order to test enzymatic activity	+	* To make Horseradish Peroxidase Assay in order to test enzymatic activity in UV-vis Spectrometer.

	==Procedure==		==Procedure==

Keyun Wang: /* Procedure */

2012-09-25T04:11:20Z

Procedure

←Older revision		Revision as of 04:11, 25 September 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
-	* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18]Melissa's Notebook]] for the final concentration of stock solution for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.	+	* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for the final concentration of stock solution for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.
		+	* Samples mixed with altering concentration of H2O2 was made and analyzed under UV-vis spectrometer. The concentration of AAP(4-aminoantipyrine) was set at 2.5mM, 4-Iodophenol was set at 18mM, and horseradish peroxidase at 2.3uM. The concentration of H2O2 was changed from 1.7mM to 106.25uM.
		+	* In the next set of samples, concentration of H2O2 is held constant. The concentration of AAP was altered from 1.25mM to 156.25uM. The concentration of other solutions were kept the same.
		+	* For results obtained from UV-vis spectrometer, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] for graph of Absorbance versus Time of enzymatic activity of HRP in Fixed [AAP] and varied [H2O2], and in Fixed [H2O2] and varied [AAP]

		+	==Notes==
		+	* The above two sets of samples were run to determine the effect on enzymatic function of function of horseradish peroxidase under different concentrations of AAP and H2O2.
		+	* It was discovered that when concentration of AAP is held constant, the enzymatic activity decreases once the concentration of H2O2 increases. This could be concluded by comparing the slope of each sample with different concentrations of H2O2.
		+	* It was discovered that when concentration of H2O2 is held constant, the enzymatic activity increases as the concentration of AAP increases. The results can be shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18\|Melissa's Notebook]] in graph Absorbance over time of Enzymatic Activity of HRP in Fixed [H2O2] and Varied [AAP] by comparing the slopes of each sample run.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
-	\|}	+

	__NOTOC__		__NOTOC__

Keyun Wang: /* Procedure */

2012-09-25T03:53:39Z

Procedure

←Older revision		Revision as of 03:53, 25 September 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
-	*	+	* For details on making stock solutions, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/18]Melissa's Notebook]] for the final concentration of stock solution for horseradish peroxidase, sodium phosphate buffer, 4-Aminoantipyrine, and 4-Iodophenol.

Keyun Wang at 17:41, 19 September 2012

2012-09-19T17:41:42Z

←Older revision		Revision as of 17:41, 19 September 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
-	* ~~[[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/18&action=edit\|need to explain this later]]~~	+	*

Keyun Wang at 17:39, 19 September 2012

2012-09-19T17:39:45Z

←Older revision		Revision as of 17:39, 19 September 2012
Line 10:		Line 10:

	==Procedure==		==Procedure==
		+	* [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/18&action=edit\|need to explain this later]]

Keyun Wang at 02:04, 19 September 2012

2012-09-19T02:04:12Z

←Older revision		Revision as of 02:04, 19 September 2012
Line 1:		Line 1:
	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here...~~	+	* To make Horseradish Peroxidase Assay in order to test enzymatic activity
		+
		+	==Procedure==

Keyun Wang: Autocreate 2012/09/18 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

2012-09-19T01:54:04Z

Autocreate 2012/09/18 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__