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		<title>User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/12 - Revision history</title>
		<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;action=history</link>
		<description>Revision history for this page on the wiki</description>
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			<title>Keyun Wang at 16:07, 5 October 2012</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=632785&amp;oldid=prev</link>
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				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 16:07, 5 October 2012&lt;/td&gt;
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		&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 8:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 8:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Purpose==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Purpose==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To check if solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced fibers&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To check if solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced fibers&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To mix supernatant &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;of sample &lt;/del&gt;with &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;fiber &lt;/del&gt;pellet with different concentration of Tris buffer at pH 10 and check re-suspension&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To mix supernatant with pellet &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;in each sample &lt;/ins&gt;with &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;the addition of &lt;/ins&gt;different concentration of Tris buffer at pH 10 and check re-suspension&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:55, 17 September 2012 (EDT)''':I'm confused by this. are you adding tris to the pelletonly, to just the supernatant or to both?&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;* Prepare the ratio 80 Au/BSA sample for peak shift testing next week&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:55, 17 September 2012 (EDT)''':explain what hte peak shift experiemnt is.&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of Au/BSA solutions with different ratios were remade from ratio 80 to ratio 170. For details with the volume, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. The sets of solutions were incubated in 85°C for four hours. After four hours of incubation, 1mL of supernatant were measured for absorption activity under UV-vis from 700nm to 200nm. &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;For details of the results, see &lt;/del&gt;[[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;.&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of Au/BSA solutions with different ratios were remade from ratio 80 to ratio 170. For details with the volume, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. The sets of solutions were incubated in 85°C for four hours. After four hours of incubation, 1mL of supernatant were measured for absorption activity under UV-vis from 700nm to 200nm. &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;See &lt;/ins&gt;[[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;for &lt;/ins&gt;details &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;on absorption of each Au/BSA solutions after 4 hours of incubation&lt;/ins&gt;.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':masses and volumes and ratios. can reference the calculations and precise &lt;/del&gt;details &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;are in Dhea's notebook&lt;/del&gt;.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 100mM Tris buffer at pH 10 were diluted to 50mM Tris buffer at pH 10 with distilled water. 1M Tris buffer were made, pH were adjusted to pH 10 with the addition of 1M HCl as stock solution stored in fridge. 500mM Tris buffer at pH 10 were made by diluting from 1M Tris buffer. &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Please refer to &lt;/ins&gt;[[User:&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Melissa Novy/Notebook/CHEM-571/2012/09/12&lt;/ins&gt;|&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Melissa's Notebook&lt;/ins&gt;]] &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;for details on &lt;/ins&gt;the &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;calculation on the actual &lt;/ins&gt;and theoretical mass &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;and concentration obtained for 100mM Tris buffer&lt;/ins&gt;.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 100mM Tris buffer at pH 10 were diluted to 50mM Tris buffer at pH 10 with distilled water. 1M Tris buffer were made, pH were adjusted to pH 10 with the addition of 1M HCl as stock solution stored in fridge. 500mM Tris buffer at pH 10 were made by diluting from 1M Tris buffer.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Absorbency were measured using UV-vis spectrometer for the sets of Au/BSA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]]. 1mL of each sample were used for measurement. For details of the absorbance, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;for the absorbance obtained for Au/BSA sample after 4 hours of incubation&lt;/ins&gt;. &amp;nbsp;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''&lt;/del&gt;[[User:&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Abigail E. Miller&lt;/del&gt;|&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Abigail E. Miller&lt;/del&gt;]] &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;10:58, 17 September 2012 (EDT)''':masses and volumes and ratios. can reference &lt;/del&gt;the &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;calculations &lt;/del&gt;and &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;rpecise details - &lt;/del&gt;theoretical mass&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;, acutal mass, etc.&lt;/del&gt;.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;on the absorbance versus wavelength &lt;/ins&gt;data &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;on each Au/BSA sample&lt;/ins&gt;. &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;It was concluded that none of &lt;/ins&gt;the &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;solution were resuspended with the purple fibrous pellet, and the previous experiments cannot be repeated. Tris buffer mixture with Au/BSA resolution remained inconclusive.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Absorbency were measured using UV-vis spectrometer for the sets of Au/BSA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]]. 1mL of each sample were used for measurement. For details of the absorbance, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;. &lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''': show data here too&lt;/del&gt;. &amp;nbsp;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;.&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':show &lt;/del&gt;data &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;here too&lt;/del&gt;. &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;what conclusions can you draw from &lt;/del&gt;the &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;data?&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Notes==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Notes==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* New sets of solutions were taken out of the incubator. Fibers were formed in all samples. It was suspected that the ratios prepared were wrong and all ratios are predicted to be above 133 Au/BSA. Supernatant were clear and fibers were formed closely together as pellet.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* New sets of solutions were taken out of the incubator. Fibers were formed in all samples. It was suspected that the ratios prepared were wrong and all ratios are predicted to be above 133 Au/BSA. Supernatant were clear and fibers were formed closely together as pellet.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced homogeneous solutions at lower ratio and fibers at higher ratios of Au/BSA &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;according &lt;/del&gt;to &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;predicted.&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced homogeneous solutions at lower ratio and fibers at higher ratios of Au/BSA &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;as previous experiments performed by other researchers. It was concluded that the ratios of Au/BSA calculations were correct, and that the procedure were reproducible. For details of the solution, please refer &lt;/ins&gt;to [[User:&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12&lt;/ins&gt;|&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Dhea's notebook&lt;/ins&gt;]] &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;for the appearance of solution for a better understanding of the structural formation &lt;/ins&gt;of fibers &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;and appearance of pellet and supernatant after incubation. &lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;*'''&lt;/del&gt;[[User:&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Abigail E. Miller&lt;/del&gt;|&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Abigail E. Miller&lt;/del&gt;]] &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;10:59, 17 September 2012 (EDT)''':be specific. what was predicted? photos &lt;/del&gt;of &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;solutions? what ratios produced &lt;/del&gt;fibers&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;? what produced nothing? what produced homogenous solutions?&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
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&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|}&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|}&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
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			<pubDate>Fri, 05 Oct 2012 16:07:22 GMT</pubDate>			<dc:creator>Keyun Wang</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Abigail E. Miller: /* Procedure */</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=626799&amp;oldid=prev</link>
			<description>&lt;p&gt;&lt;span class=&quot;autocomment&quot;&gt;Procedure&lt;/span&gt;&lt;/p&gt;

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				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 14:59, 17 September 2012&lt;/td&gt;
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&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''': show data here too. &amp;nbsp;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''': show data here too. &amp;nbsp;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':show data here too. conclusions &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;form &lt;/del&gt;data?&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':show data here too. &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;what &lt;/ins&gt;conclusions &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;can you draw from the &lt;/ins&gt;data?&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Notes==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Notes==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
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			<pubDate>Mon, 17 Sep 2012 14:59:41 GMT</pubDate>			<dc:creator>Abigail E. Miller</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Abigail E. Miller: /* Notes */</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=626798&amp;oldid=prev</link>
			<description>&lt;p&gt;&lt;span class=&quot;autocomment&quot;&gt;Notes&lt;/span&gt;&lt;/p&gt;

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				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 14:59, 17 September 2012&lt;/td&gt;
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&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* New sets of solutions were taken out of the incubator. Fibers were formed in all samples. It was suspected that the ratios prepared were wrong and all ratios are predicted to be above 133 Au/BSA. Supernatant were clear and fibers were formed closely together as pellet.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* New sets of solutions were taken out of the incubator. Fibers were formed in all samples. It was suspected that the ratios prepared were wrong and all ratios are predicted to be above 133 Au/BSA. Supernatant were clear and fibers were formed closely together as pellet.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced homogeneous solutions at lower ratio and fibers at higher ratios of Au/BSA according to predicted.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced homogeneous solutions at lower ratio and fibers at higher ratios of Au/BSA according to predicted.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;#160;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:59, 17 September 2012 (EDT)''':be specific. what was predicted? photos of solutions? what ratios produced fibers? what produced nothing? what produced homogenous solutions?&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|}&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|}&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;__NOTOC__&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;__NOTOC__&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
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			<pubDate>Mon, 17 Sep 2012 14:59:04 GMT</pubDate>			<dc:creator>Abigail E. Miller</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Abigail E. Miller: /* Procedure */</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=626797&amp;oldid=prev</link>
			<description>&lt;p&gt;&lt;span class=&quot;autocomment&quot;&gt;Procedure&lt;/span&gt;&lt;/p&gt;

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				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 14:58, 17 September 2012&lt;/td&gt;
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		&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 15:&lt;/td&gt;
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&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of Au/BSA solutions with different ratios were remade from ratio 80 to ratio 170. For details with the volume, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. The sets of solutions were incubated in 85°C for four hours. After four hours of incubation, 1mL of supernatant were measured for absorption activity under UV-vis from 700nm to 200nm. For details of the results, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]].&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Sets of Au/BSA solutions with different ratios were remade from ratio 80 to ratio 170. For details with the volume, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. The sets of solutions were incubated in 85°C for four hours. After four hours of incubation, 1mL of supernatant were measured for absorption activity under UV-vis from 700nm to 200nm. For details of the results, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]].&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':masses and volumes and ratios. can reference the calculations and precise details are in Dhea's notebook.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 100mM Tris buffer at pH 10 were diluted to 50mM Tris buffer at pH 10 with distilled water. 1M Tris buffer were made, pH were adjusted to pH 10 with the addition of 1M HCl as stock solution stored in fridge. 500mM Tris buffer at pH 10 were made by diluting from 1M Tris buffer.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 100mM Tris buffer at pH 10 were diluted to 50mM Tris buffer at pH 10 with distilled water. 1M Tris buffer were made, pH were adjusted to pH 10 with the addition of 1M HCl as stock solution stored in fridge. 500mM Tris buffer at pH 10 were made by diluting from 1M Tris buffer.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':masses and volumes and ratios. can reference the calculations and rpecise details - theoretical mass, acutal mass, etc..&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Absorbency were measured using UV-vis spectrometer for the sets of Au/BSA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]]. 1mL of each sample were used for measurement. For details of the absorbance, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. &amp;nbsp;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Absorbency were measured using UV-vis spectrometer for the sets of Au/BSA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]]. 1mL of each sample were used for measurement. For details of the absorbance, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. &amp;nbsp;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''': show data here too. &lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results.&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results.&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:58, 17 September 2012 (EDT)''':show data here too. conclusions form data?&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Notes==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Notes==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
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&lt;/table&gt;</description>
			<pubDate>Mon, 17 Sep 2012 14:58:03 GMT</pubDate>			<dc:creator>Abigail E. Miller</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Abigail E. Miller: /* Purpose */</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=626796&amp;oldid=prev</link>
			<description>&lt;p&gt;&lt;span class=&quot;autocomment&quot;&gt;Purpose&lt;/span&gt;&lt;/p&gt;

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				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 14:55, 17 September 2012&lt;/td&gt;
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		&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 9:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 9:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To check if solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced fibers&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To check if solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced fibers&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To mix supernatant of sample with fiber pellet with different concentration of Tris buffer at pH 10 and check re-suspension&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* To mix supernatant of sample with fiber pellet with different concentration of Tris buffer at pH 10 and check re-suspension&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:55, 17 September 2012 (EDT)''':I'm confused by this. are you adding tris to the pelletonly, to just the supernatant or to both?&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Prepare the ratio 80 Au/BSA sample for peak shift testing next week&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* Prepare the ratio 80 Au/BSA sample for peak shift testing next week&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:55, 17 September 2012 (EDT)''':explain what hte peak shift experiemnt is.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;!-- diff generator: internal 2013-05-20 16:56:13 --&gt;
&lt;/table&gt;</description>
			<pubDate>Mon, 17 Sep 2012 14:55:20 GMT</pubDate>			<dc:creator>Abigail E. Miller</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Keyun Wang at 20:36, 12 September 2012</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=625652&amp;oldid=prev</link>
			<description>&lt;p&gt;&lt;/p&gt;

			&lt;table style=&quot;background-color: white; color:black;&quot;&gt;
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			&lt;tr valign='top'&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 20:36, 12 September 2012&lt;/td&gt;
			&lt;/tr&gt;
		&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 12:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 12:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==Procedure==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;* Sets of Au/BSA solutions with different ratios were remade from ratio 80 to ratio 170. For details with the volume, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. The sets of solutions were incubated in 85°C for four hours. After four hours of incubation, 1mL of supernatant were measured for absorption activity under UV-vis from 700nm to 200nm. For details of the results, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]].&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;* 100mM Tris buffer at pH 10 were diluted to 50mM Tris buffer at pH 10 with distilled water. 1M Tris buffer were made, pH were adjusted to pH 10 with the addition of 1M HCl as stock solution stored in fridge. 500mM Tris buffer at pH 10 were made by diluting from 1M Tris buffer.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;* Absorbency were measured using UV-vis spectrometer for the sets of Au/BSA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]]. 1mL of each sample were used for measurement. For details of the absorbance, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]]. &lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;* 4mL of 133 Au/BSA solution made on 09/11/12 were mixed with 1mL of 50mM Tris buffer at pH 10, yielding a total of 10mM tris buffer in solution. 4mL of 136 Au/BSA solution were mixed with 1mL of 100mM Tris buffer at pH 10, yielding a total of 20mM tris buffer in solution. And 4mL of 138 Au/BSA solution were mixed with 1mL of 500M Tris buffer at pH 10, yielding a total of 100mM tris buffer in solution. All three samples were lightly shaken and let to re-suspend for 30 minutes. Absorbance for the three samples were taken after 30 minutes and after 90 minutes. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/12|Dhea's Notebook]] for results.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;==Notes==&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;* New sets of solutions were taken out of the incubator. Fibers were formed in all samples. It was suspected that the ratios prepared were wrong and all ratios are predicted to be above 133 Au/BSA. Supernatant were clear and fibers were formed closely together as pellet.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;* Sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced homogeneous solutions at lower ratio and fibers at higher ratios of Au/BSA according to predicted.&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;!-- diff generator: internal 2013-05-20 16:56:13 --&gt;
&lt;/table&gt;</description>
			<pubDate>Wed, 12 Sep 2012 20:36:51 GMT</pubDate>			<dc:creator>Keyun Wang</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Keyun Wang at 18:40, 12 September 2012</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=625595&amp;oldid=prev</link>
			<description>&lt;p&gt;&lt;/p&gt;

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				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;←Older revision&lt;/td&gt;
				&lt;td colspan='2' style=&quot;background-color: white; color:black;&quot;&gt;Revision as of 18:40, 12 September 2012&lt;/td&gt;
			&lt;/tr&gt;
		&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 1:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 1:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;{|{{table}} width=&amp;quot;800&amp;quot;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;{|{{table}} width=&amp;quot;800&amp;quot;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|style=&amp;quot;background-color: #EEE&amp;quot;|[[Image:owwnotebook_icon.png|128px]]&amp;lt;span style=&amp;quot;font-size:22px;&amp;quot;&amp;gt; &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Project name&lt;/del&gt;&amp;lt;/span&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|style=&amp;quot;background-color: #EEE&amp;quot;|[[Image:owwnotebook_icon.png|128px]]&amp;lt;span style=&amp;quot;font-size:22px;&amp;quot;&amp;gt; &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Experimental Biological Chemistry I&lt;/ins&gt;&amp;lt;/span&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|style=&amp;quot;background-color: #F2F2F2&amp;quot; align=&amp;quot;center&amp;quot;|&amp;lt;html&amp;gt;&amp;lt;img src=&amp;quot;/images/9/94/Report.png&amp;quot; border=&amp;quot;0&amp;quot; /&amp;gt;&amp;lt;/html&amp;gt; [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]&amp;lt;br /&amp;gt;{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|&amp;lt;html&amp;gt;&amp;lt;img src=&amp;quot;/images/c/c3/Resultset_previous.png&amp;quot; border=&amp;quot;0&amp;quot; /&amp;gt;&amp;lt;/html&amp;gt;[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&amp;lt;html&amp;gt;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/html&amp;gt;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]&amp;lt;html&amp;gt;&amp;lt;img src=&amp;quot;/images/5/5c/Resultset_next.png&amp;quot; border=&amp;quot;0&amp;quot; /&amp;gt;&amp;lt;/html&amp;gt;}}&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|style=&amp;quot;background-color: #F2F2F2&amp;quot; align=&amp;quot;center&amp;quot;|&amp;lt;html&amp;gt;&amp;lt;img src=&amp;quot;/images/9/94/Report.png&amp;quot; border=&amp;quot;0&amp;quot; /&amp;gt;&amp;lt;/html&amp;gt; [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]&amp;lt;br /&amp;gt;{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|&amp;lt;html&amp;gt;&amp;lt;img src=&amp;quot;/images/c/c3/Resultset_previous.png&amp;quot; border=&amp;quot;0&amp;quot; /&amp;gt;&amp;lt;/html&amp;gt;[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&amp;lt;html&amp;gt;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/html&amp;gt;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]&amp;lt;html&amp;gt;&amp;lt;img src=&amp;quot;/images/5/5c/Resultset_next.png&amp;quot; border=&amp;quot;0&amp;quot; /&amp;gt;&amp;lt;/html&amp;gt;}}&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;| colspan=&amp;quot;2&amp;quot;|&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;| colspan=&amp;quot;2&amp;quot;|&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Entry title&lt;/del&gt;==&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;==&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;Purpose&lt;/ins&gt;==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;-&lt;/td&gt;&lt;td style=&quot;background: #ffa; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* &lt;del class=&quot;diffchange diffchange-inline&quot;&gt;Insert content here...&lt;/del&gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;* &lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;To check if solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11|2012/09/11]] has produced fibers&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;* To mix supernatant of sample with fiber pellet with different concentration of Tris buffer at pH 10 and check re-suspension&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;* Prepare the ratio 80 Au/BSA sample for peak shift testing next week&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot;&gt;&amp;nbsp;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;background: #cfc; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&lt;ins style=&quot;color: red; font-weight: bold; text-decoration: none;&quot;&gt;==Procedure==&lt;/ins&gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background: #eee; color:black; font-size: smaller;&quot;&gt;&lt;div&gt;&amp;lt;!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
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&lt;/table&gt;</description>
			<pubDate>Wed, 12 Sep 2012 18:40:23 GMT</pubDate>			<dc:creator>Keyun Wang</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
		<item>
			<title>Keyun Wang: Autocreate 2012/09/12 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I</title>
			<link>http://openwetware.org/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12&amp;diff=625588&amp;oldid=prev</link>
			<description>&lt;p&gt;Autocreate 2012/09/12 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;{|{{table}} width=&amp;quot;800&amp;quot;&lt;br /&gt;
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			<pubDate>Wed, 12 Sep 2012 18:32:51 GMT</pubDate>			<dc:creator>Keyun Wang</dc:creator>			<comments>http://openwetware.org/wiki/User_talk:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/12</comments>		</item>
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