User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11 - Revision history

Keyun Wang at 15:58, 5 October 2012

2012-10-05T15:58:19Z

←Older revision		Revision as of 15:58, 5 October 2012
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	==Procedure==		==Procedure==
-	* Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction.	+	* Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions at concentrations used for 2012/09/04. The two samples were placed in incubator for an hour to check for possible reaction.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:52, 17 September 2012 (EDT)''':what stock solution? concentrations? when were they prepared? volumes and masses?	+
	* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].		* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].
	* After an hour in the incubator, the 120 Au/BSA ratio solution turned to transparent, and the purple fibers formed in 60 Au/BSA ratio solutions. New sets of HAuCl4 and BSA solutions at different ratios were made with below amounts:		* After an hour in the incubator, the 120 Au/BSA ratio solution turned to transparent, and the purple fibers formed in 60 Au/BSA ratio solutions. New sets of HAuCl4 and BSA solutions at different ratios were made with below amounts:
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	* Sets of sample of Au/BSA with different ratios appeared gold in color after four hours in incubation. This indicates HAuCl4 has failed to react with BSA in solution.		* Sets of sample of Au/BSA with different ratios appeared gold in color after four hours in incubation. This indicates HAuCl4 has failed to react with BSA in solution.
	* Au/BSA solutions mixed in tris buffer had a pellet at the bottom of the 50mL falcon tube, supernatent were carefully taken. For some samples the pellets were disturbed when transferring. To obtain pure supernatent, the falcon tubes were centrifuged.		* Au/BSA solutions mixed in tris buffer had a pellet at the bottom of the 50mL falcon tube, supernatent were carefully taken. For some samples the pellets were disturbed when transferring. To obtain pure supernatent, the falcon tubes were centrifuged.
-		+	* From the absorbance measurements, we concluded that all Au/BSA ratios worked successfully, results appeared identical to the results obtained from past experiments. All ratios for Au/BSA were concluded to be correct.
-	*~~'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:53, 17 September 2012 (EDT)''':it is unclear what conclusions you drew from~~ the absorbance measurements?	+

Abigail E. Miller: /* Notes */

2012-09-17T14:53:23Z

Notes

←Older revision		Revision as of 14:53, 17 September 2012
Line 54:		Line 54:
	* Sets of sample of Au/BSA with different ratios appeared gold in color after four hours in incubation. This indicates HAuCl4 has failed to react with BSA in solution.		* Sets of sample of Au/BSA with different ratios appeared gold in color after four hours in incubation. This indicates HAuCl4 has failed to react with BSA in solution.
	* Au/BSA solutions mixed in tris buffer had a pellet at the bottom of the 50mL falcon tube, supernatent were carefully taken. For some samples the pellets were disturbed when transferring. To obtain pure supernatent, the falcon tubes were centrifuged.		* Au/BSA solutions mixed in tris buffer had a pellet at the bottom of the 50mL falcon tube, supernatent were carefully taken. For some samples the pellets were disturbed when transferring. To obtain pure supernatent, the falcon tubes were centrifuged.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:53, 17 September 2012 (EDT)''':it is unclear what conclusions you drew from the absorbance measurements?

Abigail E. Miller: /* Procedure */

2012-09-17T14:52:37Z

Procedure

←Older revision		Revision as of 14:52, 17 September 2012
Line 12:		Line 12:
	==Procedure==		==Procedure==
	* Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction.		* Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:52, 17 September 2012 (EDT)''':what stock solution? concentrations? when were they prepared? volumes and masses?
	* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].		* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].
	* After an hour in the incubator, the 120 Au/BSA ratio solution turned to transparent, and the purple fibers formed in 60 Au/BSA ratio solutions. New sets of HAuCl4 and BSA solutions at different ratios were made with below amounts:		* After an hour in the incubator, the 120 Au/BSA ratio solution turned to transparent, and the purple fibers formed in 60 Au/BSA ratio solutions. New sets of HAuCl4 and BSA solutions at different ratios were made with below amounts:

Keyun Wang: /* Procedure */

2012-09-11T19:46:37Z

Procedure

←Older revision		Revision as of 19:46, 11 September 2012
Line 11:		Line 11:

	==Procedure==		==Procedure==
-	* ~~New sets of samples were made with a different sets of calculations..([[add calculations]]).~~ Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction.	+	* Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction.
	* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].		* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].
-	* ~~[[Checking the sample after~~ an hour...]]	+	* After an hour in the incubator, the 120 Au/BSA ratio solution turned to transparent, and the purple fibers formed in 60 Au/BSA ratio solutions. New sets of HAuCl4 and BSA solutions at different ratios were made with below amounts:
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|''HAuCl4/BSA ratio''''
		+	\| align="center" style="background:#f0f0f0;"\|''BSA[uL]''''
		+	\| align="center" style="background:#f0f0f0;"\|''HAuCl4[uL]''''
		+	\| align="center" style="background:#f0f0f0;"\|''H2O[mL]''''
		+	\|-
		+	\| 60\|\|165\|\|32.2\|\|3.803
		+	\|-
		+	\| 80\|\|165\|\|43.0\|\|3.792
		+	\|-
		+	\| 100\|\|165\|\|53.7\|\|3.781
		+	\|-
		+	\| 120\|\|165\|\|64.5\|\|3.771
		+	\|-
		+	\| 128\|\|165\|\|68.7\|\|3.766
		+	\|-
		+	\| 130\|\|165\|\|69.8\|\|3.765
		+	\|-
		+	\| 132\|\|165\|\|70.9\|\|3.764
		+	\|-
		+	\| 133\|\|165\|\|71.4\|\|3.764
		+	\|-
		+	\| 134\|\|165\|\|72.0\|\|3.763
		+	\|-
		+	\| 136\|\|165\|\|73.0\|\|3.762
		+	\|-
		+	\| 138\|\|165\|\|74.1\|\|3.761
		+	\|-
		+	\| 140\|\|165\|\|75.2\|\|3.760
		+	\|-
		+	\| 160\|\|165\|\|85.9\|\|3.749
		+	\|-
		+	\| 170\|\|165\|\|91.3\|\|3.744
		+	\|-
		+	\|}

	==Notes==		==Notes==

Keyun Wang at 18:05, 11 September 2012

2012-09-11T18:05:02Z

←Older revision		Revision as of 18:05, 11 September 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here~~...	+	* Check if newly made sample on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]] has reacted after 4 hours in the incubator at 85°C
		+	* Measure UV absorbance on samples that were diluted in Tris buffer in different concentration and pH
		+
		+	==Procedure==
		+	* New sets of samples were made with a different sets of calculations..([[add calculations]]). Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction.
		+	* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05\|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11\|Melissa's Notebook]].
		+	* [[Checking the sample after an hour...]]
		+
		+	==Notes==
		+	* Sets of sample of Au/BSA with different ratios appeared gold in color after four hours in incubation. This indicates HAuCl4 has failed to react with BSA in solution.
		+	* Au/BSA solutions mixed in tris buffer had a pellet at the bottom of the 50mL falcon tube, supernatent were carefully taken. For some samples the pellets were disturbed when transferring. To obtain pure supernatent, the falcon tubes were centrifuged.

Keyun Wang: Autocreate 2012/09/11 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

2012-09-11T17:39:01Z

Autocreate 2012/09/11 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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