User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/11: Difference between revisions
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==Procedure== | ==Procedure== | ||
* | * Two samples, one at 120 Au/BSA and another at 60 Au/BSA were made with stock solutions. The two samples were placed in incubator for an hour to check for possible reaction. | ||
* UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11|Melissa's Notebook]]. | * UV absorbance were taken from samples that were mixed with Tris buffer from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05|2012/09/05]]. Sample at the ratio 134, 136, 138, 140, 160, 170 Au to BSA were centrifuged at 4000rpm, 7°C, for 5 minutes to ensure separation of supernatant and pallet. 500uL of each supernatant were used in each quartz cuvette. Each sample were run from 800nm to 200nm. The graph Absorbance versus Wavelength were plotted and shown in [[User:Melissa Novy/Notebook/CHEM-571/2012/09/11|Melissa's Notebook]]. | ||
* [[ | * After an hour in the incubator, the 120 Au/BSA ratio solution turned to transparent, and the purple fibers formed in 60 Au/BSA ratio solutions. New sets of HAuCl4 and BSA solutions at different ratios were made with below amounts: | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|''HAuCl4/BSA ratio'''' | |||
| align="center" style="background:#f0f0f0;"|''BSA[uL]'''' | |||
| align="center" style="background:#f0f0f0;"|''HAuCl4[uL]'''' | |||
| align="center" style="background:#f0f0f0;"|''H2O[mL]'''' | |||
|- | |||
| 60||165||32.2||3.803 | |||
|- | |||
| 80||165||43.0||3.792 | |||
|- | |||
| 100||165||53.7||3.781 | |||
|- | |||
| 120||165||64.5||3.771 | |||
|- | |||
| 128||165||68.7||3.766 | |||
|- | |||
| 130||165||69.8||3.765 | |||
|- | |||
| 132||165||70.9||3.764 | |||
|- | |||
| 133||165||71.4||3.764 | |||
|- | |||
| 134||165||72.0||3.763 | |||
|- | |||
| 136||165||73.0||3.762 | |||
|- | |||
| 138||165||74.1||3.761 | |||
|- | |||
| 140||165||75.2||3.760 | |||
|- | |||
| 160||165||85.9||3.749 | |||
|- | |||
| 170||165||91.3||3.744 | |||
|- | |||
|} | |||
==Notes== | ==Notes== |
Revision as of 12:46, 11 September 2012
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Purpose
Procedure
Notes
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