User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05 - Revision history

Keyun Wang at 15:56, 5 October 2012

Keyun Wang — Fri, 05 Oct 2012 15:56:28 GMT

←Older revision		Revision as of 15:56, 5 October 2012
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	* Resuspending Au/BSA solutions in Tris buffer		* Resuspending Au/BSA solutions in Tris buffer
	*# The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.		*# The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.
-	*# The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer.	+	*# The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer. The details of buffer-pallet mix is shown in table below:
-		+
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:50, 17 September 2012 (EDT)''': what masses were used to make the "new" solutions? what volumes used to dilute? what date was the "old" set of solutions made and heated? what had you done to them?	+
-		+
-	*# The details of buffer-pallet mix is shown in table below:	+
	{\|		{\|
	\|-		\|-
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	==Notes==		==Notes==
-	* The new sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04\|2012/09/04]] failed to react. The solutions after heating appeared yellow in color. This indicates the BSA failed to react with the HAuCl4 in solution.	+	* The new sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04\|2012/09/04]] failed to react. The solutions after heating appeared yellow in color. This indicates the BSA failed to react with the HAuCl4 in solution.
-		+	* In this experiment, the tris buffer the fiber were found in previous research to mix back together and create a homologous mixture. However, after the mixing of tris buffer at pH 8.0 and 10.0, no mixture were observed. The procedure from previous experiments cannot be repeated. More concentrations and pH of tris buffer were planned to try out next week for pellet resuspension.
-	*~~'''[[User:Abigail E. Miller\|Abigail E~~. ~~Miller]] 10:51~~, ~~17 September 2012 (EDT)''':what happened with~~ the tris buffer and ~~fiber experiment?~~	+

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Abigail E. Miller: /* Notes */

Abigail E. Miller — Mon, 17 Sep 2012 14:51:08 GMT

Notes

←Older revision		Revision as of 14:51, 17 September 2012
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	* The new sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04\|2012/09/04]] failed to react. The solutions after heating appeared yellow in color. This indicates the BSA failed to react with the HAuCl4 in solution.		* The new sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04\|2012/09/04]] failed to react. The solutions after heating appeared yellow in color. This indicates the BSA failed to react with the HAuCl4 in solution.

		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:51, 17 September 2012 (EDT)''':what happened with the tris buffer and fiber experiment?

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Abigail E. Miller: /* Procedure */

Abigail E. Miller — Mon, 17 Sep 2012 14:50:18 GMT

Procedure

←Older revision		Revision as of 14:50, 17 September 2012
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	*# The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.		*# The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.
	*# The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer.		*# The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:50, 17 September 2012 (EDT)''': what masses were used to make the "new" solutions? what volumes used to dilute? what date was the "old" set of solutions made and heated? what had you done to them?
		+
	*# The details of buffer-pallet mix is shown in table below:		*# The details of buffer-pallet mix is shown in table below:
	{\|		{\|

Keyun Wang: /* Procedure */

Keyun Wang — Sat, 08 Sep 2012 19:42:56 GMT

Procedure

←Older revision		Revision as of 19:42, 8 September 2012
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	\|}		\|}
	*# The tris buffer and Au/BSA solution were mixed gently and let sit for 30 minutes.		*# The tris buffer and Au/BSA solution were mixed gently and let sit for 30 minutes.
-	*# s	+	*# After 30 minutes, 500uL of each sample were loaded into quark cuvette and placed into UV-Vis to measure absorbance from 800nm to 200mm.
		+	*# Graph were plotted with Absorbance versus Wavelength, for details of absorbance from each sample, see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/05\|Dhea Patel's Lab Notebook]]

	==Notes==		==Notes==

Keyun Wang: /* Procedure */

Keyun Wang — Wed, 05 Sep 2012 19:59:48 GMT

Procedure

←Older revision		Revision as of 19:59, 5 September 2012
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	==Procedure==		==Procedure==
	* A new sets of solutions of BSA and HAuCl4 mixture were made. New stock solutions of HAuCl4 and BSA were also made.		* A new sets of solutions of BSA and HAuCl4 mixture were made. New stock solutions of HAuCl4 and BSA were also made.
-	* The old sets of solutions of Au/BSA at different ratios were...	+	* 100mM tris buffer at pH 8.0 and pH 10.0 were diluted with serial dilution to four different concentrations: 10mM, 1mM, 100uM, and 10uM, respectively.
		+	* Resuspending Au/BSA solutions in Tris buffer
		+	*# The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.
		+	*# The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer.
		+	*# The details of buffer-pallet mix is shown in table below:
		+	{\|
		+	\|-
		+	! Ratio of Au/BSA Pallet
		+	! Tris Buffer Concentrations [mM]
		+	! Tris Buffer pH
		+	\|-
		+	\| 132
		+	\| 10
		+	\| 10
		+	\|-
		+	\| 133
		+	\| 1
		+	\| 10
		+	\|-
		+	\| 134
		+	\| 0.1
		+	\| 10
		+	\|-
		+	\| 136
		+	\| 0.01
		+	\| 10
		+	\|-
		+	\| 138
		+	\| 10
		+	\| 8
		+	\|-
		+	\| 140
		+	\| 1
		+	\| 8
		+	\|-
		+	\| 160
		+	\| 0.1
		+	\| 8
		+	\|-
		+	\| 170
		+	\| 0.01
		+	\| 8
		+	\|}
		+	*# The tris buffer and Au/BSA solution were mixed gently and let sit for 30 minutes.
		+	*# s

	==Notes==		==Notes==

Keyun Wang: /* Entry title */

Keyun Wang — Wed, 05 Sep 2012 19:22:15 GMT

Entry title

←Older revision		Revision as of 19:22, 5 September 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here~~...	+	* To resuspend gold nano-particle pallets with Tris buffer.
		+	* To determine the best concentration and pH of Tris buffer for resuspension via measuring the absorbance of resuspended solutions throughout time.
		+	* To make new sets of Au/BSA solutions with varies ratios.
		+
		+	==Procedure==
		+	* A new sets of solutions of BSA and HAuCl4 mixture were made. New stock solutions of HAuCl4 and BSA were also made.
		+	* The old sets of solutions of Au/BSA at different ratios were...
		+
		+	==Notes==
		+	* The new sets of solutions made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04\|2012/09/04]] failed to react. The solutions after heating appeared yellow in color. This indicates the BSA failed to react with the HAuCl4 in solution.


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-	\|}	+

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Keyun Wang at 18:09, 5 September 2012

Keyun Wang — Wed, 05 Sep 2012 18:09:54 GMT

←Older revision		Revision as of 18:09, 5 September 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Keyun Wang: Autocreate 2012/09/05 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

Keyun Wang — Wed, 05 Sep 2012 18:08:19 GMT

Autocreate 2012/09/05 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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