User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05: Difference between revisions

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==Procedure==
==Procedure==
* A new sets of solutions of BSA and HAuCl4 mixture were made. New stock solutions of HAuCl4 and BSA were also made.  
* A new sets of solutions of BSA and HAuCl4 mixture were made. New stock solutions of HAuCl4 and BSA were also made.  
* The old sets of solutions of Au/BSA at different ratios were...
* 100mM tris buffer at pH 8.0 and pH 10.0 were diluted with serial dilution to four different concentrations: 10mM, 1mM, 100uM, and 10uM, respectively.
* Resuspending Au/BSA solutions in Tris buffer
*# The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.
*# The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer.
*# The details of buffer-pallet mix is shown in table below:
{|
|-
! Ratio of Au/BSA Pallet
! Tris Buffer Concentrations [mM]
! Tris Buffer pH
|-
| 132
| 10
| 10
|-
| 133
| 1
| 10
|-
| 134
| 0.1
| 10
|-
| 136
| 0.01
| 10
|-
| 138
| 10
| 8
|-
| 140
| 1
| 8
|-
| 160
| 0.1
| 8
|-
| 170
| 0.01
| 8
|}
*# The tris buffer and Au/BSA solution were mixed gently and let sit for 30 minutes.  
*# s


==Notes==
==Notes==

Revision as of 12:59, 5 September 2012

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Purpose

  • To resuspend gold nano-particle pallets with Tris buffer.
  • To determine the best concentration and pH of Tris buffer for resuspension via measuring the absorbance of resuspended solutions throughout time.
  • To make new sets of Au/BSA solutions with varies ratios.

Procedure

  • A new sets of solutions of BSA and HAuCl4 mixture were made. New stock solutions of HAuCl4 and BSA were also made.
  • 100mM tris buffer at pH 8.0 and pH 10.0 were diluted with serial dilution to four different concentrations: 10mM, 1mM, 100uM, and 10uM, respectively.
  • Resuspending Au/BSA solutions in Tris buffer
    1. The old sets of solutions of Au/BSA at different ratios were spin in centrifuge at 3000rpm for 5 minutes at room temperature.
    2. The supernatant were poured out. Pallet of different Au/BSA ratios were resuspended in different concentrations and different pH of Tris buffer.
    3. The details of buffer-pallet mix is shown in table below:
Ratio of Au/BSA Pallet Tris Buffer Concentrations [mM] Tris Buffer pH
132 10 10
133 1 10
134 0.1 10
136 0.01 10
138 10 8
140 1 8
160 0.1 8
170 0.01 8
    1. The tris buffer and Au/BSA solution were mixed gently and let sit for 30 minutes.
    2. s

Notes

  • The new sets of solutions made on 2012/09/04 failed to react. The solutions after heating appeared yellow in color. This indicates the BSA failed to react with the HAuCl4 in solution.
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