User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04: Difference between revisions
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* Solutions of Au/BSA at various ratios were remade. New stock solutions were remade. Solutions were wrapped around with aluminum foil and placed in incubator at 85°C for five hours. Incubator were cooled to room temperature until the next day. | * Solutions of Au/BSA at various ratios were remade. New stock solutions were remade. Solutions were wrapped around with aluminum foil and placed in incubator at 85°C for five hours. Incubator were cooled to room temperature until the next day. | ||
* 1.2114g of Tris buffer was mixed with 100mL of distilled water to make 100mM of tris buffer solution. Two sets of solutions were made. In each 100mL tris buffer, each sample was titrated to pH 8.0 and pH 10.0 with the addition of 1M HCl, respectively. | * 1.2114g of Tris buffer was mixed with 100mL of distilled water to make 100mM of tris buffer solution. Two sets of solutions were made. In each 100mL tris buffer, each sample was titrated to pH 8.0 and pH 10.0 with the addition of 1M HCl, respectively. | ||
* New sets of Au/BSA solutions at various ratios that were pre-made before were placed in centrifuge and were attempted to spin at 4000rpm for 5 minutes in room temperature. However, two glass test tubes that were placed on the outskirts of centrifuge shattered during the process. All solutions were then transferred into falcon tubes. The Au/BSA solution at ratio 130 and 128 were disposed due to mix of broken glass. Some purple fibers were stuck on glass test tubes while being transferred to falcon tube and are lost during the transfer. | |||
==Notes== | ==Notes== |
Revision as of 12:28, 5 September 2012
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