User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04

Purpose

• To run UV-Vis analysis on Au/BSA solutions at various ratios.
• To remake solutions that did not give expected results.
• To make Tris Buffer at 100mM at pH 8.0 and 10.0

Procedure

• The absorbance of each sample were taken by UV-vis spectrophotometer. Graph were made with absorbance versus wavelength. See Dhea Patel's Lab Notebook on 2012/09/04 for details.

Notes

• The original sets of solutions did not form nano-particles, solutions appeared yellow in color. It was predicted that the HAuCl4 reacted with gold specula during the transfer.
• A new set of solutions with different Au/BSA ratios were made beforehand at the same ratio and concentrations. For details of the concentrations, see entry from 2012/08/29
• The new set of solutions with different ratios of Au and BSA appeared purpose in color. The 60 Au/BSA solution appeared cloudy and white. The 80 Au/BSA solution appeared as a purpose homogenous solution. The Au/BSA solution with other ratios are transparent with purpose fiber formations.