User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29 - Revision history

Keyun Wang: /* Procedure */

2012-10-16T18:17:24Z

Procedure

←Older revision		Revision as of 18:17, 16 October 2012
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	* Bovine Serum Albumin stock solution was prepared at 15.806uM.		* Bovine Serum Albumin stock solution was prepared at 15.806uM.
	* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.		* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
-	* The HAuCl4 stock wre measured by combining 0.0971g of HAuCl4 in 25mL of water. The BSA stock were calculated by combining 0.0105g of BSA in 1mL of water. The formula weight of HAuCl4 is 393.83g/mol and formula weight of bSA is 66430 g/mol. Please see [[Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/2\|Dhea's notebook]] for details of the calculation to determine the concentration of the two stock solutions.	+	* The HAuCl4 stock wre measured by combining 0.0971g of HAuCl4 in 25mL of water. The BSA stock were calculated by combining 0.0105g of BSA in 1mL of water. The formula weight of HAuCl4 is 393.83g/mol and formula weight of bSA is 66430 g/mol. Please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/29\|Dhea's notebook]] for details of the calculation to determine the concentration of the two stock solutions.
	* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.		* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
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Keyun Wang at 15:41, 5 October 2012

2012-10-05T15:41:44Z

←Older revision		Revision as of 15:41, 5 October 2012
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	* Bovine Serum Albumin stock solution was prepared at 15.806uM.		* Bovine Serum Albumin stock solution was prepared at 15.806uM.
	* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.		* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
-	*~~'''~~[[~~User:Abigail E. Miller~~\|~~Abigail E. Miller~~]] ~~10:36, 17 September 2012 (EDT)''':what masses and volumes were used? what are the calculations for those and~~ for ~~determining~~ the ~~dilutions below? these need~~ to ~~be demonstrated atleast once in your notebook and then you can refer back to them when you repeat~~.	+	* The HAuCl4 stock wre measured by combining 0.0971g of HAuCl4 in 25mL of water. The BSA stock were calculated by combining 0.0105g of BSA in 1mL of water. The formula weight of HAuCl4 is 393.83g/mol and formula weight of bSA is 66430 g/mol. Please see [[Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/2\|Dhea's notebook]] for details of the calculation to determine the concentration of the two stock solutions.
-		+
	* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.		* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
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	==Notes==		==Notes==
-	* ~~Various~~ ratios of Au/BSA Solutions appeared yellow and transparent when mixed.	+	* All ratios of Au/BSA Solutions appeared yellow and transparent when mixed.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''': all ratios or just some of them?	+
	* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.		* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.

Abigail E. Miller: /* Notes */

2012-09-17T14:39:36Z

Notes

←Older revision		Revision as of 14:39, 17 September 2012
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	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''': all ratios or just some of them?		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''': all ratios or just some of them?
	* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.		* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''':what did you do with the solutions? heat or leave at room temperature?	+
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Abigail E. Miller: /* Notes */

2012-09-17T14:39:21Z

Notes

←Older revision		Revision as of 14:39, 17 September 2012
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	==Notes==		==Notes==
-	* Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed.	+	* Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''': all ratios or just some of them?
	* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.		* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''':what did you do with the solutions? heat or leave at room temperature?
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->


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Abigail E. Miller: /* Procedure */

2012-09-17T14:36:35Z

Procedure

←Older revision		Revision as of 14:36, 17 September 2012
Line 13:		Line 13:
	* Bovine Serum Albumin stock solution was prepared at 15.806uM.		* Bovine Serum Albumin stock solution was prepared at 15.806uM.
	* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.		* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:36, 17 September 2012 (EDT)''':what masses and volumes were used? what are the calculations for those and for determining the dilutions below? these need to be demonstrated atleast once in your notebook and then you can refer back to them when you repeat.
		+
	* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.		* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
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Keyun Wang: /* Procedure */

2012-09-03T02:24:12Z

Procedure

←Older revision		Revision as of 02:24, 3 September 2012
Line 91:		Line 91:
	\| 3.3278		\| 3.3278
	\|}		\|}
-	* 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at ~~__°C~~, and left in room temperature until the following week.	+	* 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at 85°C, and left in room temperature until the following week.

	==Notes==		==Notes==

Keyun Wang: /* Procedure */

2012-09-03T02:21:17Z

Procedure

←Older revision		Revision as of 02:21, 3 September 2012
Line 11:		Line 11:

	==Procedure==		==Procedure==
-	* Bovine Serum Albumin stock solution was prepared at ~~15uM~~.	+	* Bovine Serum Albumin stock solution was prepared at 15.806uM.
-	* Chloroauric Acid (HAuCl4) stock solution was prepared at ~~10mM~~.	+	* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
	* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.		* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
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Keyun Wang: /* Entry title */

2012-09-03T02:16:49Z

Entry title

←Older revision		Revision as of 02:16, 3 September 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Purpose==
-	* ~~Insert content here...~~	+	* To test the absorbance in solutions with various ratio of gold nanoparticles (AU) to BSA (Bovine Serum Albumin)
		+	* To discover trends in the increase of Au/BSA ratios

		+	==Procedure==
		+	* Bovine Serum Albumin stock solution was prepared at 15uM.
		+	* Chloroauric Acid (HAuCl4) stock solution was prepared at 10mM.
		+	* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
		+	{\|
		+	\|-
		+	! Au/BSA ratio
		+	! BSA[uL]
		+	! HAuCl4[uL]
		+	! H2O added[mL]
		+	\|-
		+	\| 60
		+	\| 253.1
		+	\| 147.9
		+	\| 3.5990
		+	\|-
		+	\| 80
		+	\| 253.1
		+	\| 197.2
		+	\| 3.5497
		+	\|-
		+	\| 100
		+	\| 253.1
		+	\| 246.6
		+	\| 3.5004
		+	\|-
		+	\| 120
		+	\| 253.1
		+	\| 295.9
		+	\| 3.4511
		+	\|-
		+	\| 128
		+	\| 253.1
		+	\| 315.6
		+	\| 3.4313
		+	\|-
		+	\| 130
		+	\| 253.1
		+	\| 320.5
		+	\| 3.4264
		+	\|-
		+	\| 132
		+	\| 253.1
		+	\| 325.4
		+	\| 3.4215
		+	\|-
		+	\| 133
		+	\| 253.1
		+	\| 327.9
		+	\| 3.4190
		+	\|-
		+	\| 134
		+	\| 253.1
		+	\| 330.4
		+	\| 3.4166
		+	\|-
		+	\| 136
		+	\| 253.1
		+	\| 335.3
		+	\| 3.4116
		+	\|-
		+	\| 138
		+	\| 253.1
		+	\| 340.2
		+	\| 3.4067
		+	\|-
		+	\| 140
		+	\| 253.1
		+	\| 345.2
		+	\| 3.4018
		+	\|-
		+	\| 160
		+	\| 253.1
		+	\| 394.5
		+	\| 3.3524
		+	\|-
		+	\| 170
		+	\| 253.1
		+	\| 419.1
		+	\| 3.3278
		+	\|}
		+	* 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at __°C, and left in room temperature until the following week.

		+	==Notes==
		+	* Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed.
		+	* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
-	\|}	+

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Keyun Wang at 19:51, 2 September 2012

2012-09-02T19:51:50Z

←Older revision		Revision as of 19:51, 2 September 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-

Keyun Wang: Autocreate 2012/08/29 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

2012-09-02T19:50:52Z

Autocreate 2012/08/29 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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