User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Bovine Serum Albumin stock solution was prepared at 15.806uM. | * Bovine Serum Albumin stock solution was prepared at 15.806uM. | ||
* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM. | * Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM. | ||
* The HAuCl4 stock wre measured by combining 0.0971g of HAuCl4 in 25mL of water. The BSA stock were calculated by combining 0.0105g of BSA in 1mL of water. The formula weight of HAuCl4 is 393.83g/mol and formula weight of bSA is 66430 g/mol. Please see [[Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/ | * The HAuCl4 stock wre measured by combining 0.0971g of HAuCl4 in 25mL of water. The BSA stock were calculated by combining 0.0105g of BSA in 1mL of water. The formula weight of HAuCl4 is 393.83g/mol and formula weight of bSA is 66430 g/mol. Please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/29|Dhea's notebook]] for details of the calculation to determine the concentration of the two stock solutions. | ||
* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details. | * BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details. | ||
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Latest revision as of 21:56, 26 September 2017
Experimental Biological Chemistry I | Main project page Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
Notes
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