User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29: Difference between revisions
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* Bovine Serum Albumin stock solution was prepared at 15.806uM. | * Bovine Serum Albumin stock solution was prepared at 15.806uM. | ||
* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM. | * Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM. | ||
* The HAuCl4 stock wre measured by combining 0.0971g of HAuCl4 in 25mL of water. The BSA stock were calculated by combining 0.0105g of BSA in 1mL of water. The formula weight of HAuCl4 is 393.83g/mol and formula weight of bSA is 66430 g/mol. Please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/29|Dhea's notebook]] for details of the calculation to determine the concentration of the two stock solutions. | |||
* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details. | * BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details. | ||
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* 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at | * 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at 85°C, and left in room temperature until the following week. | ||
==Notes== | ==Notes== | ||
* | * All ratios of Au/BSA Solutions appeared yellow and transparent when mixed. | ||
* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects. | * Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects. | ||
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Revision as of 11:17, 16 October 2012
 Experimental Biological Chemistry I
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