User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29

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(Notes)
(Notes)
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*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''': all ratios or just some of them?
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''': all ratios or just some of them?
* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.
* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.
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*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:39, 17 September 2012 (EDT)''':what did you do with the solutions? heat or leave at room temperature?
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Revision as of 10:39, 17 September 2012

Experimental Biological Chemistry I Main project page
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Purpose

  • To test the absorbance in solutions with various ratio of gold nanoparticles (AU) to BSA (Bovine Serum Albumin)
  • To discover trends in the increase of Au/BSA ratios

Procedure

  • Bovine Serum Albumin stock solution was prepared at 15.806uM.
  • Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
  • Abigail E. Miller 10:36, 17 September 2012 (EDT):what masses and volumes were used? what are the calculations for those and for determining the dilutions below? these need to be demonstrated atleast once in your notebook and then you can refer back to them when you repeat.
  • BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
Au/BSA ratio BSA[uL] HAuCl4[uL] H2O added[mL]
60 253.1 147.9 3.5990
80 253.1 197.2 3.5497
100 253.1 246.6 3.5004
120 253.1 295.9 3.4511
128 253.1 315.6 3.4313
130 253.1 320.5 3.4264
132 253.1 325.4 3.4215
133 253.1 327.9 3.4190
134 253.1 330.4 3.4166
136 253.1 335.3 3.4116
138 253.1 340.2 3.4067
140 253.1 345.2 3.4018
160 253.1 394.5 3.3524
170 253.1 419.1 3.3278
  • 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at 85°C, and left in room temperature until the following week.

Notes

  • Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed.
  • Abigail E. Miller 10:39, 17 September 2012 (EDT): all ratios or just some of them?
  • Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.



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