User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29

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Revision as of 22:21, 2 September 2012

Experimental Biological Chemistry I

To test the absorbance in solutions with various ratio of gold nanoparticles (AU) to BSA (Bovine Serum Albumin)
To discover trends in the increase of Au/BSA ratios

Bovine Serum Albumin stock solution was prepared at 15.806uM.
Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.

Au/BSA ratio	BSA[uL]	HAuCl4[uL]	H2O added[mL]
60	253.1	147.9	3.5990
80	253.1	197.2	3.5497
100	253.1	246.6	3.5004
120	253.1	295.9	3.4511
128	253.1	315.6	3.4313
130	253.1	320.5	3.4264
132	253.1	325.4	3.4215
133	253.1	327.9	3.4190
134	253.1	330.4	3.4166
136	253.1	335.3	3.4116
138	253.1	340.2	3.4067
140	253.1	345.2	3.4018
160	253.1	394.5	3.3524
170	253.1	419.1	3.3278

40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at __°C, and left in room temperature until the following week.

Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed.
Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.

@@ Line 11: / Line 11: @@
 ==Procedure==
-* Bovine Serum Albumin stock solution was prepared at 15uM.
+* Bovine Serum Albumin stock solution was prepared at 15.806uM.
-* Chloroauric Acid (HAuCl4) stock solution was prepared at 10mM.
+* Chloroauric Acid (HAuCl4) stock solution was prepared at 9.862mM.
 * BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
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