User:Keyun Wang/Notebook/Experimental Biological Chemistry I

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==Project Description/Abstract==
==Project Description/Abstract==
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* Place short description of project or notes regarding this project
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* Gold nanoparticles has shown to form by mixingof HAuCl4 and BSA. From past experiments, Bakshi, et al. showed that different mole ratios of Au/protein samples yielded samples that either had purple solutions, or purple fibers, or both. The goal of the project is to find out the fiber formation in relation to mole ratio of gold to protein, and how environmental factors such as pH, ionic strength, isoelectric point of protein affects gold nanoparticle formation.
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* It is suspected that the mole ratio of Au/protein that forms aggregated protein nanoparticles differ depending on the different protein used. In this project, proteins such as Horseradish peroxidase, Adenosine deaminase, Lysozyme, and Bovine serum albumin were used with different mole ratios of HAuCl<sub>4</sub> ranging from 10 to 500 to see the effects of gold nanoparticle formation for different proteins.
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* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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* It was found that through the same procedure and within the same mole ratio of gold to protein, ADA protein yielded the least amount of gold in solution, while BSA protein yielded the most amount of gold in solution.
==Notes==
==Notes==
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* Place some notes here for visitors
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* Project scheduled to go on until December 2012.
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* Example: This project is currently on hold until further notice.
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Project Description/Abstract

  • Gold nanoparticles has shown to form by mixingof HAuCl4 and BSA. From past experiments, Bakshi, et al. showed that different mole ratios of Au/protein samples yielded samples that either had purple solutions, or purple fibers, or both. The goal of the project is to find out the fiber formation in relation to mole ratio of gold to protein, and how environmental factors such as pH, ionic strength, isoelectric point of protein affects gold nanoparticle formation.
  • It is suspected that the mole ratio of Au/protein that forms aggregated protein nanoparticles differ depending on the different protein used. In this project, proteins such as Horseradish peroxidase, Adenosine deaminase, Lysozyme, and Bovine serum albumin were used with different mole ratios of HAuCl4 ranging from 10 to 500 to see the effects of gold nanoparticle formation for different proteins.
  • It was found that through the same procedure and within the same mole ratio of gold to protein, ADA protein yielded the least amount of gold in solution, while BSA protein yielded the most amount of gold in solution.

Notes

  • Project scheduled to go on until December 2012.

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