User:Kersten S. Rabe

Contact Info

Kersten S. Rabe - Half of him

• Dipl. Biochem. Kersten S. Rabe
• TU Dortmund
• Otto-Hahn Strasse 6
• BCMT
• 44227 Dortmund, Germany
• Email me through OpenWetWare

I work as a Post-Doc in the Niemeyer lab at Dortmund University. In Dec. 2009 I finished my time as Doktorand with "summa cum laude" ("Ausgezeichnet")

Education

• 2009-present Post-Doc with Prof. Niemeyer
• 2005-2009 phD work in the group of Prof. Niemeyer at University of Dortmund, Germany, on Protein-DNA-conjugates. Title of thesis: "Herstellung rekombinanter Proteine für die Synthese von DNA-Protein-Konjugaten". Mark: summa cum laude ("Ausgezeichnet")
• 2004 eight month research assistant at the group of Prof. Sprinzl, University of Bayreuth, Germany
• 2004 Diploma (Biochemistry), Group of Prof. Sprinzl, University of Bayreuth, Germany
• Research semester, Group of Dr. Ubbink, Protein Chemistry (former Metallo Protein Research), University of Leiden, Netherlands: "The Transient Complex of Cytochrome c and Cytochrome f - Investigated by Heteronuclear NMR"
• 1999-2004, Graduate studies in Biochemistry, University of Bayreuth
• 1999 Intermediate Diploma ("Vordiplom")
• 1997-1999, Undergraduate studies in Biochemistry, University of Bayreuth
• 1996/97 Thirteen month of civilian service
• 1996 finished Abitur (final secondary school examination) at the Graf-Anton-Günther-Schule, Oldenburg, Germany

Research interests

1. Synthetic biology
2. Nanobiotechnology
3. Chemical Biology

Artwork

I worked a lot with the thermostable P450 CYP119A1 therefore I prepared a little movie for my PhD defence. Media:CYP119A1.ogg In this movie you can see the P450 enzyme CYP119A1 with the inhibitor phenylimidazol bound to the active site.

Publications

First author

April 2009 I could design the coverpicture for ChemBioChem, where I could show my rendering skills again. [1]

1. Rabe KS and Niemeyer CM. Hydrogels: Gene jelly. Nat Mater. 2009 May;8(5):370-2. DOI:10.1038/nmat2434 |
2. Rabe KS, Spengler M, Erkelenz M, Müller J, Gandubert VJ, Hayen H, and Niemeyer CM. Screening for cytochrome p450 reactivity by harnessing catalase as reporter enzyme. Chembiochem. 2009 Mar 2;10(4):751-7. DOI:10.1002/cbic.200800750 |

3. Rabe KS, Feldkamp U, and Niemeyer CM.

   Semisynthetic DNA-protein conjugates for fabrication of nucleic acid based nanostructures.

   AIP Conf. Proc. 2009; 1062(1) 19-32. doi: 10.1063/1.3012299 Permalink

4. Rabe KS, Gandubert VJ, Spengler M, Erkelenz M, and Niemeyer CM. Engineering and assaying of cytochrome P450 biocatalysts. Anal Bioanal Chem. 2008 Nov;392(6):1059-73. DOI:10.1007/s00216-008-2248-9 |
5. Rabe KS, Kiko K, and Niemeyer CM. Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius. Chembiochem. 2008 Feb 15;9(3):420-5. DOI:10.1002/cbic.200700450 |

Co-author

6. Svahn MG, Rabe KS, Barger G, El-Andaloussi S, Simonson OE, Didier B, Olivier R, Dumy P, Brandén LJ, Niemeyer CM, and Smith CIE.

   High-Throughput Identification of Combinatorial Ligands for DNA Delivery in Cell Culture.

   AIP Conf. Proc. 2009; 1062(1) 73-88. doi: 10.1063/1.3012306 Permalink

7. Agafonov DE, Rabe KS, Grote M, Voertler CS, and Sprinzl M. C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1. Chembiochem. 2006 Feb;7(2):330-6. DOI:10.1002/cbic.200500358 |
8. Agafonov DE, Rabe KS, Grote M, Huang Y, and Sprinzl M. The esterase from Alicyclobacillus acidocaldarius as a reporter enzyme and affinity tag for protein biosynthesis. FEBS Lett. 2005 Apr 11;579(10):2082-6. DOI:10.1016/j.febslet.2005.02.059 |
9. Crowley PB, Rabe KS, Worrall JA, Canters GW, and Ubbink M. The ternary complex of cytochrome f and cytochrome c: identification of a second binding site and competition for plastocyanin binding. Chembiochem. 2002 Jun 3;3(6):526-33. DOI:10.1002/1439-7633(20020603)3:6<526::AID-CBIC526>3.0.CO;2-N |

Patents

SPRINZL MATHIAS (DE); AGAFONOV DMITRY (DE); RABE KERSTEN (DE) ESTERASES FOR MONITORING PROTEIN BIOSYNTHESIS IN VITRO Publication number: WO2006082059

SPRINZL MATHIAS (DE); AGAFONOV DMITRY (DE); RABE KERSTEN (DE) CELL-FREE TRANSLATION SYSTEM FOR THE PRODUCTION OF (CHEMICALLY) MODIFIED PROTEINS Publication number: WO2006082058

Useful links

• Introductory tutorial
• OpenWetWare help pages