User:Kenji Doering/Notebook/Flow Cell Set Up/2011/07/26: Difference between revisions
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* Measure out 2 mg of Ovalbumin | * Measure out 2 mg of Ovalbumin | ||
* Add the 2 mg of Ovalbumin the the 1.5 mL aliquot, and dilute to the 1 mL mark with DI water | * Add the 2 mg of Ovalbumin the the 1.5 mL aliquot, and dilute to the 1 mL mark with DI water -the sample should now have a concentration of 2 mg/mL | ||
* Add 100 microliters of the sample to five separate 500 microliter aliquots. | * Add 100 microliters of the sample to five separate 500 microliter aliquots. | ||
* Dilute each of the aliquots with an extra 100 microliters of DI water | * Dilute each of the aliquots with an extra 100 microliters of DI water -the new samples are all at 1 mg/mL now. | ||
* Using the DynaPro measure the hydrodynamic radius of each of the samples over a time of [insert time here] | * Using the DynaPro measure the hydrodynamic radius of each of the samples over a time of [insert time here] at temperatures of 20,34,48,62,76 degrees Celcius, respectively. | ||
* Record and Analyze results. | * Record and Analyze results. | ||
Revision as of 14:17, 26 July 2011
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Practicing with the Dynamic Light Scattering Machine using Ovalbumin chicken egg-whitesMachine: DynaPro Titan TC
This experiment is just for practice use of the Dynamic Light Scattering System.
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