mm/dd/yy
- Outline of experiments/ tasks
Task 1
> Line item 1
| Reagent | Volume | Conc.
|
| reagent 1 | vol 1 | conc 1
|
| reagent 2 | vol 2 | conc 2
|
| dH2O | vol 3 |
|
| | total vol | --> Reaction conditions
|
Lipo transfection
> Details
| Wells | Plasmid | DNA | Volume | Lipo | Opti-MEM (total)
|
| 1 | name | 2 μg | ### μL | 4 μL | 500 μL
|
| 2 | name | " | ### μL | " | "
|
| 3 | name | " | ### μL | " | "
|
| 4 | name | " | ### μL | " | "
|
| 5 | name | " | ### μL | " | "
|
| 6 | name | " | ### μL | " | "
|
> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min.
> Add DNA to 250 μL Opti-MEM
> Add DNA mix to Lipo mix --> R.T./ 20 min.
> Remove 500 μL medium from each well; Add 500 μL complexes to each well (4 ml med. each); Grow cells at 37°C
> Refresh medium after 5 hours (ab-free)
Fugene transfection
> Details
| Wells | Plasmid | DNA | Volume | Fugene | Opti-MEM
|
| 1-6 | name | 200 ng | ### μL | 1.8 μL | 18.2 μL
|
| 7-12 | name | " | ### μL | " | "
|
| 13-18 | name | " | ### μL | " | "
|
| 19-24 | name | " | ### μL | " | "
|
> Master mixes (4, x6): 10.8 μL Fugene + 109.2 μL Opti-MEM --> R.T/ 5 min.
> Add 6x vol DNA --> R.T./ 20 min.
> Add 20 μL complexes to each well (1 ml med. each); Grow cells at 37°C overnight
| 
Project name
Main project page
