User:Karmella Haynes/Notebook/Polycomb project/2013/01/09: Difference between revisions

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'''Thaw cells'''<br>
'''Thaw cells'''<br>
* Quick thaw. Add to 7 mL FBS-tet-free
* Quick thaw. Add to 7 mL FBS-tet-free
* Spin for 5 min., 1000 rpm, r.t.
* Spin for 3 min., 1000 rpm, r.t.
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s

Revision as of 19:29, 9 January 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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01/09/13

  • Thaw new cells: U2OS Flp-in TRex, HEK293 Flp-in TRex

Thaw cells

  • Quick thaw. Add to 7 mL FBS-tet-free
  • Spin for 3 min., 1000 rpm, r.t.
    • U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
    • HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
  • Grow in small flat bottom flasks


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