User:Karmella Haynes/Notebook/Polycomb project/2013/01/09: Difference between revisions
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* | * Thaw new cells: U2OS Flp-in TRex, HEK293 Flp-in TRex | ||
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''' | '''Thaw cells'''<br> | ||
* Quick thaw. Add to 7 mL FBS-tet-free | |||
* Spin for 3 min., 1000 rpm, r.t. | |||
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s | |||
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s | |||
* Grow in small flat bottom flasks | |||
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Revision as of 19:29, 9 January 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
01/09/13
Thaw cells
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