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01/09/13
- Thaw new cells: U2OS Flp-in TRex, HEK293 Flp-in TRex
Thaw cells
- Quick thaw. Add to 7 mL FBS-tet-free
- Spin for 3 min., 1000 rpm, r.t.
- U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
- HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
- Grow in small flat bottom flasks
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