User:Karmella Haynes/Notebook/Polycomb project/2011/02/25

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02/25/11

  • ✓ Cell culture: thaw KAH126-1
  • ✓ RT-PCR planning: design primers for new putative targets based on old Nanostring raw data



RT-PCR planning
> Go back to Nanostring results and pick all candidates up-regulated by Pc-TFs (at least 2.0 fold induction, dox+/dox-)
> Will test primers on KAH-126-1 cDNA (-/+ dox)
> Will follow up with other samples and control lines