User:Karmella Haynes/Notebook/Polycomb project/2010/01/13

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01/13/10

  • ✓ Polycomb-ATF lines: expand selected clones in 6-well dishes (1 clone/ well)
  • ✓ Protein preps (for Western): from Dox+, selected Polycomb-ATF clones


Protein preps
> Polycomb-ATF clones
> KAH126-129, 132, 133 Dox+ from 24-well plates
--> Let KAH130, 131 grow for > 2 days before prepping (low cell density) (Prepped on Saturday 1/16)
> Use 100 μL RIPA + protease inhibitor/ sample
--> Incubate @ 4°C, rotating, overnight


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