User:Karmella Haynes/Notebook/PcTF Genomics/2015/08/18: Difference between revisions

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==08/18/15==
==08/18/15==
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* Line item 1
* Western - Castone PAGE & blot
 


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'''Line item 1'''<br>
'''Western - Castone PAGE & blot'''<br>
> Samples
* Trouble-shooting: saw some indication of signal last time; BSA is recommended for modified proteins
** Re-run the Gal4-EED sample set
** Use BSA-based blocking buffer for downstream steps
 
 


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Revision as of 11:38, 18 August 2015

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08/18/15

  • Western - Castone PAGE & blot



Western - Castone PAGE & blot

  • Trouble-shooting: saw some indication of signal last time; BSA is recommended for modified proteins
    • Re-run the Gal4-EED sample set
    • Use BSA-based blocking buffer for downstream steps


Reagent Volume
reagent 1 # μL
reagent 2 #
reagent 3 #
reagent 4 #
dH2O #
  # μL

--> Reaction conditions