User:Karmella Haynes/Notebook/PcTF Genomics/2015/08/12: Difference between revisions
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'''TRANSFER''' | '''TRANSFER''' | ||
Electroblot | |||
* Load both at same time, "feet" facing inward | * Load both at same time, "feet" facing inward | ||
* Transblot Turbo transfer pack, mini format, 0.2 um PVDF | * Transblot Turbo transfer pack, mini format, 0.2 um PVDF | ||
* Bio-Rad Transblot Turbo: Bio-Rad Protocol, Mixed MW, 2.5A, 25V, 7 min (slot A, upper) | * Bio-Rad Transblot Turbo: Bio-Rad Protocol, Mixed MW, 2.5A, 25V, 7 min (slot A, upper) | ||
* Note: Wow! worked super well and only took 7 min! However, did not see 10 kD band on the membrane, but 15 kD is there (histones) | |||
Poncaeu stain | |||
* Rinsed blot in di-water and wahed in 1x Poncaeu for ~15 min. | |||
* Rinsed off excess with di-water | |||
* Note: Pxi4 imager not set up correctly, troubleshoot tomorrow with Solo Pyon | |||
Revision as of 16:01, 13 August 2015
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08/12/15
Castone Western - gel and transfers
General:
Protein sample prep:
Loading - Gel 1:
Loading - Gel 2:
Run gels: 120 V, 50 min.
Electroblot
Poncaeu stain
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