User:Karmella Haynes/Notebook/PcTF Genomics/2015/08/12: Difference between revisions
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'''Castone Western - gel and transfers'''<br> | '''Castone Western - gel and transfers'''<br> | ||
* Transfections of dCas-Histone + gRNA plasmids were done on 6/25 and 6/27 | |||
* Protein was prepped using the standard procedure describe at: | |||
* Samples: | |||
# Luc14: H340_MV11 + g034_pSPgRNA | |||
# Luc14: H340_MV11 (no gRNA) | |||
# Luc14: H360_MV11 + g034_pSPgRNA | |||
# Luc14: H360_MV11 (no gRNA) | |||
# Luc14: H380_MV11 + g034_pSPgRNA | |||
# Luc14: H380_MV11 (no gRNA) | |||
# Gal4EED-luc: H340_MV11 + g034_pSPgRNA | |||
# Gal4EED-luc: H340_MV11 (no gRNA) | |||
# Gal4EED-luc: H360_MV11 + g034_pSPgRNA | |||
# Gal4EED-luc: H360_MV11 (no gRNA) | |||
# Gal4EED-luc: H380_MV11 + g034_pSPgRNA | |||
# Gal4EED-luc: H380_MV11 (no gRNA) | |||
* Run two gels (1-6 and 7-12) | |||
--> Reaction conditions | --> Reaction conditions | ||
Revision as of 15:53, 12 August 2015
 Pc-TF Genomics
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08/12/15
Castone Western - gel and transfers
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