User:Karmella Haynes/Notebook/PcTF Genomics/2015/06/25: Difference between revisions
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# H340_MV11 + pSP### | # H340_MV11 + pSP### | ||
# | # H360_MV11 + pSP### | ||
# | # H380_MV11 + pSP### | ||
# | # H3116_MV11 + pSP### | ||
# | # H3136_MV11 + pSP### | ||
# pSP### (negative) | # pSP### (negative) | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Trfxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Trfxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Well</u> || <u>H3#_MV11 (1.0 μg)</u> || <u>pSP# (1.0 μg)</u> || PLUS rgt. || Lipo LTX | | <u>Well</u> || <u>H3#_MV11 (1.0 μg)</u> || <u>pSP# (1.0 μg)</u> || OptiMEM || PLUS rgt. || Lipo LTX | ||
|- | |- | ||
| 1 || # | | 1 || # || # || 570.0 || 2.5 || 7.5 | ||
|- | |- | ||
| 2 || # | | 2 || # || # || 570.0 || 2.5 || 7.5 | ||
|- | |- | ||
| 3 || # | | 3 || # || # || 570.0 || 2.5 || 7.5 | ||
|- | |- | ||
| 4 || # | | 4 || # || # || 570.0 || 2.5 || 7.5 | ||
|- | |- | ||
| 5 || # | | 5 || # || # || 570.0 || 2.5 || 7.5 | ||
|- | |- | ||
| 6 || # | | 6 || # || # || 570.0 || 2.5 || 7.5 | ||
|} | |} | ||
--> | * Pre-warm ps-free DMEM | ||
* Make 20 μL plasmid/dH<sub>2</sub>O mixes in sterile 1.5 ml tubes | |||
* Add OptiMEM | |||
* Add PLUS --> 5 min./ RT | |||
* Add Lipo LTX --> 30 min./ RT | |||
* Refresh growth medium w/ ps-free DMEM | |||
* Add 600 μL complexes | |||
* Incubate overnight | |||
Revision as of 17:05, 25 June 2015
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mm/dd/yy
Cas-tone - transfections
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