User:Karmella Haynes/Notebook/PcTF Genomics/2015/06/25: Difference between revisions
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<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* | * Cas-tone - cell transfections | ||
---- | ---- | ||
''' | '''Cas-tone - transfections'''<br> | ||
* Final H3#_MV11 builds verified on 6/24/15 | |||
* Co-transfect H3#_MV11 with pSP-guide RNA plasmid (built by Ben Nyer) | |||
* Use Luc14 cells (from Rene) | |||
{| class="wikitable" border="0" cellspacing="3" <!-- | # H340_MV11 + pSP### | ||
# H340_MV11 + pSP### | |||
# H340_MV11 + pSP### | |||
# H340_MV11 + pSP### | |||
# H340_MV11 + pSP### | |||
# pSP### (negative) | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Trfxn. table --> | |||
|-valign="top" | |-valign="top" | ||
| <u> | | <u>Well</u> || <u>H3#_MV11 (1.0 μg)</u> || <u>pSP# (1.0 μg)</u> || PLUS rgt. || Lipo LTX | ||
|- | |- | ||
| | | 1 || # μL || # μL || || μL | ||
|- | |- | ||
| | | 2 || # | ||
|- | |- | ||
| | | 3 || # | ||
|- | |- | ||
| | | 4 || # | ||
|- | |- | ||
| | | 5 || # | ||
|- | |- | ||
| | | 6 || # | ||
|} | |} | ||
Revision as of 16:58, 25 June 2015
 Pc-TF Genomics
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mm/dd/yy
Cas-tone - transfections
--> Reaction conditions
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