User:Karmella Haynes/Notebook/PcTF Genomics/2015/03/20: Difference between revisions
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'''PAGE - glucagon & insulin'''<br> | '''PAGE - glucagon & insulin'''<br> | ||
> Sample prep | > From David Barclay's pancreas cell project | ||
* Glucagon - | |||
> Gel lanes | '''Sample prep''' | ||
# | * Loading vol. 20 uL = 5.0 μL 4x Novagen sample buffer (blue) + 1.0 μL 1M DTT + 14 uL protein | ||
* Dilutions - make enough for at least two lanes plus pipetting error (x3) | |||
{| | |||
|- | |||
| Protein | Final mass | Stock μL (x3) | dH<sub>2</sub>O μL (x3) | |||
|- | |||
| Glucagon | 10.0 μg | 10.0 (30.0) | 4.0 (12.0) | |||
|- | |||
| Glucagon | 1.0 μg | 1.0 (3.0) | 13.0 (39.0) | |||
|- | |||
| Glucagon | 0.1 μg | 0.1 (0.3) | 13.9 (41.7) | |||
|} | |||
> Gel lanes (for both gels) | |||
# Page Ruler plus (15 uL) | |||
# Glucagon 10.0 μg | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | ||
Revision as of 12:23, 23 March 2015
 Pc-TF Genomics
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||
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03/20/15
PAGE - glucagon & insulin
--> Reaction conditions
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