User:Karmella Haynes/Notebook/PcTF Genomics/2015/01/28: Difference between revisions
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'''Cell plating (1/23/15)''' | '''Cell plating (1/23/15)''' | ||
* Poly lysine coating of cells (done last week) - follow protocol | * Poly-L-lysine coating of cells (done last week) - follow [http://openwetware.org/wiki/Haynes:Mamalian_IFC Mammalian IFC protocol] | ||
* Seed cells at 100k per well (aliquot in 1mL volumes into 24-well plate with poly-lysine coated cover slips on bottom) | * Seed cells at 100k per well (aliquot in 1mL volumes into 24-well plate with poly-lysine coated cover slips on bottom) | ||
* Same day - Induce cells with 1.0, 0.1, and 0.01 μg/mL dox; final volume = 2 mL | * Same day - Induce cells with 1.0, 0.1, and 0.01 μg/mL dox; final volume = 2 mL |
Revision as of 10:46, 29 January 2015
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01/28/15
Cytology of KAH126
Cell plating (1/23/15)
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